Cochliomyia hominivorax is the dipteran that causes the worst and most serious types of myiasis in animals and humans in the Americas. They are parasitic affections caused by the massive infestation of larvae of this species on the tissues of living mammals. The routine treatment uses chemosynthetic products that have high toxicity and can cause several injuries to animal and human health, besides environmental contamination. The development of more friendly alternatives to the treatment of this disease is an important knowledge gap nowadays. Citronella (Cymbopogon winterianus) is the most widely cultivated medicinal plant in tropical and subtropical regions. Essential oils have a recognised repellent and larvicidal activity against a wide variety of insects. This work aimed to evaluate the repellent, ovicidal and larvicidal efficacy of a topical commercial formulation composed of 15% (w/v) nanoemulsified Citronella essencial oil (Nano Citronela Plus®) in in vitro trials on specimens of C. hominivorax created under laboratory conditions. Nano Citronela Plus® is an oil-in-water nanoemulsion obtained by high-pressure homogenization method. The composition of tested nanoemulsion was Cymbopogon winterianus essential oil, vanillin, caprylic/capric triglyceride, polysorbate 80, sorbitan oleate, tocopheryl acetate, sodium benzoate and water. Nano Citronela Plus® demonstrated 100% lethality on eggs and first and second stage larvae of C. hominivorax. Mortality in the larvae of third stage was 69.33% and inhibited the oviposition of C. hominivorax females. Nano Citronela Plus® proved to be a possible herbal alternative for the control and treatment of myiasis caused by C. hominivorax. In vivo studies may test this hypothesis.

Cochliomyia hominivorax是导致美洲动物和人类最严重和最严重的蝇蛆病的双翅目昆虫。它们是由于该物种的幼虫大量侵染活哺乳动物的组织而引起的寄生虫病。常规治疗使用具有高毒性的化学合成产品，除了环境污染外，还会对动物和人类健康造成多种伤害。开发更友好的治疗这种疾病的替代方法是当今一个重要的知识差距。香茅（Cymbopogon Winterianus)是热带和亚热带地区栽培最广的药用植物。精油对多种昆虫具有公认的驱虫和杀幼虫活性。这项工作旨在评估由 15% (w/v) 纳米乳化香茅精油 (Nano Citronela Plus®) 组成的局部商业制剂的驱虫、杀卵和杀幼虫功效，该制剂对实验室条件下产生的C. hominvorax标本进行体外试验. Nano Citronela Plus® 是一种通过高压均质法获得的水包油纳米乳液。受试纳米乳的成分为冬青香竹精油、香兰素、辛酸/癸酸甘油三酯、聚山梨醇酯 80、脱水山梨糖醇油酸酯、生育酚乙酸酯、苯甲酸钠和水。Nano Citronela Plus® 对C. hominvorax 的卵和第一和第二阶段幼虫表现出 100% 的致死率。第三阶段幼虫的死亡率为69.33%，并抑制了C. hominivorax雌性的产卵。Nano Citronela Plus® 被证明是一种可能的草药替代品，用于控制和治疗由C. hominivorax引起的蝇蛆病。体内研究可能会检验这一假设。