Solid-state nanopores have been used extensively in biomolecular studies involving DNA and proteins. However, the interpretation of signals generated by the translocation of proteins or protein-DNA complexes remains challenging. Here, we investigate the behavior of monovalent streptavidin and the complex it forms with short biotinylated DNA over a range of nanopore sizes, salts and voltages. We describe a simple geometric model that is broadly applicable and employ it to explain observed variations in conductance blockage and dwell time with experimental conditions. The general approach developed here underscores the value of nanopore-based protein analysis and represents progress toward the interpretation of complex translocation signals.

中文翻译：

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将纳米孔信号的变化映射到蛋白质和蛋白质-DNA 构象的变化

固态纳米孔已广泛用于涉及 DNA 和蛋白质的生物分子研究。然而，对蛋白质或蛋白质-DNA 复合物易位产生的信号的解释仍然具有挑战性。在这里，我们研究了单价链霉亲和素的行为以及它与短生物素化 DNA 形成的复合物在一系列纳米孔径、盐和电压范围内。我们描述了一个广泛适用的简单几何模型，并用它来解释在实验条件下观察到的电导阻塞和停留时间的变化。这里开发的一般方法强调了基于纳米孔的蛋白质分析的价值，并代表了解释复杂易位信号的进展。