The chemical coupling of a protoplasmatic antigen from Mycobacterium avium subsp. paratubeculosis onto core-shell carboxylated particles was investigated with the aim of producing latex-protein complexes to be used in immunoagglutination assays capable of detecting bovine paratuberculosis disease. For this purpose, sensitizations were carried out using both colored and not colored carboxylated latexes as well as the protoplasmatic antigen at pH close to its isoelectric point to favor the antigenic protein to approach the particle surface. In all cases, higher fractions of proteins were chemically-bound to carboxyl groups on the surface of the particles. The assessment of the performance of the visual immunoagglutination assays consisted of evaluating 111 sera from healthy and infected bovines with Mycobacterium avium subsp. paratuberculosis. Complexes obtained from the colored latex allowed an acceptable visual discrimination between the studied positive and negative sera. Most of the positive samples showed strong to very strong agglutination and only a few samples reacted weakly, i.e. a sensitivity of 70%. The specificity of the assay, on the other hand, was 86%. Therefore, this rapid detection technique allows an easy and inexpensive identification of animals possibly infected with paratuberculosis “in situ” in the herds.

来自鸟分枝杆菌亚种的原生质抗原的化学偶联。副结核研究了核壳羧化颗粒上的核壳羧化颗粒，目的是生产乳胶蛋白复合物，用于能够检测牛副结核病的免疫凝集测定。为此，使用有色和无色羧化乳胶以及原生质抗原在接近其等电点的 pH 值下进行致敏，以有利于抗原蛋白接近颗粒表面。在所有情况下，较高比例的蛋白质与颗粒表面的羧基化学结合。视觉免疫凝集试验的性能评估包括评估来自健康和感染牛分枝杆菌亚种的111 份血清。副结核病. 从有色胶乳中获得的复合物允许在所研究的阳性和阴性血清之间进行可接受的视觉区分。大多数阳性样品表现出强至极强的凝集，只有少数样品反应微弱，即灵敏度为70%。另一方面，该测定的特异性为 86%。因此，这种快速检测技术可以轻松且廉价地识别畜群中可能“原位”感染副结核病的动物。