Science Advances ( IF 11.7 ) Pub Date : 2021-06-18 , DOI: 10.1126/sciadv.abf8630 Luciano G Morosi 1, 2 , Anabela M Cutine 1, 2 , Alejandro J Cagnoni 1, 2 , Montana N Manselle-Cocco 2 , Diego O Croci 3 , Joaquín P Merlo 1, 4 , Rosa M Morales 2 , María May 5 , Juan M Pérez-Sáez 2 , María R Girotti 4 , Santiago P Méndez-Huergo 2 , Betiana Pucci 6 , Aníbal H Gil 6 , Sergio P Huernos 6 , Guillermo H Docena 7 , Alicia M Sambuelli 6 , Marta A Toscano 2 , Gabriel A Rabinovich 2, 4, 8 , Karina V Mariño 1
Diverse immunoregulatory circuits operate to preserve intestinal homeostasis and prevent inflammation. Galectin-1 (Gal1), a β-galactoside–binding protein, promotes homeostasis by reprogramming innate and adaptive immunity. Here, we identify a glycosylation-dependent “on-off” circuit driven by Gal1 and its glycosylated ligands that controls intestinal immunopathology by targeting activated CD8+ T cells and shaping the cytokine profile. In patients with inflammatory bowel disease (IBD), augmented Gal1 was associated with dysregulated expression of core 2 β6-N-acetylglucosaminyltransferase 1 (C2GNT1) and α(2,6)-sialyltransferase 1 (ST6GAL1), glycosyltransferases responsible for creating or masking Gal1 ligands. Mice lacking Gal1 exhibited exacerbated colitis and augmented mucosal CD8+ T cell activation in response to 2,4,6-trinitrobenzenesulfonic acid; this phenotype was partially ameliorated by treatment with recombinant Gal1. While C2gnt1−/− mice exhibited aggravated colitis, St6gal1−/− mice showed attenuated inflammation. These effects were associated with intrinsic T cell glycosylation. Thus, Gal1 and its glycosylated ligands act to preserve intestinal homeostasis by recalibrating T cell immunity.
中文翻译:
通过糖基化依赖的凝集素驱动的免疫调节回路控制肠道炎症
不同的免疫调节回路起作用以保持肠道稳态并预防炎症。Galectin-1 (Gal1) 是一种 β-半乳糖苷结合蛋白,通过重新编程先天免疫和适应性免疫来促进体内平衡。在这里,我们确定了由 Gal1 及其糖基化配体驱动的糖基化依赖性“开-关”电路,该电路通过靶向活化的 CD8 + T 细胞和塑造细胞因子谱来控制肠道免疫病理学。在炎症性肠病 (IBD) 患者中,增强的 Gal1 与核心 2 β6- N-乙酰氨基葡萄糖转移酶1 ( C2GNT1 ) 和 α(2,6)-唾液酸转移酶 1 ( ST6GAL1 ) 的表达失调有关),负责产生或掩蔽 Gal1 配体的糖基转移酶。缺乏 Gal1 的小鼠表现出对 2,4,6-三硝基苯磺酸的反应加剧的结肠炎和增强的粘膜 CD8 + T 细胞活化;这种表型通过重组 Gal1 处理得到部分改善。虽然C2gnt1 -/-小鼠表现出加重的结肠炎,但St6gal1 -/-小鼠表现出减轻的炎症。这些作用与内在 T 细胞糖基化有关。因此,Gal1 及其糖基化配体通过重新校准 T 细胞免疫来维持肠道稳态。