e19148 Background: Macrophages in tumor microenvironment have been characterized as M1- and M2-polarized subtypes. Our study sought to investigate the impacts of different macrophage on lung cancer cells and the association with the survival of lung cancer patients. Methods: THP-1 monocytic cells were polarized into different macrophage subtypes M0, M1, M2a, and M2c. The transcriptomic profiling was analyzed by expression microarray for identifying M1/M2 associated gene signatures. We definitized the M1 relative genes were protective and M2 were risky genes, which were used to predict outcome in a published microarray data of lung cancers. After coculture with macrophages, the phenotypes of A549 cells were evaluated in vitro and in vivo. Results: Three gene signatures were identified based on the published patient cohorts (n = 443), including an M1-specific gene signature, M2-specific gene signature and M1/M2 combination gene signature. Patients with a high-risk gene signature exhibited shorter, median ov...

中文翻译：

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M1 和 M2 巨噬细胞亚型对肺癌进展的相反影响。

e19148 背景：肿瘤微环境中的巨噬细胞被表征为 M1 和 M2 极化亚型。我们的研究旨在探讨不同巨噬细胞对肺癌细胞的影响以及与肺癌患者生存的关系。方法：将 THP-1 单核细胞极化为不同的巨噬细胞亚型 M0、M1、M2a 和 M2c。通过表达微阵列分析转录组分析以鉴定 M1/M2 相关基因特征。我们将 M1 相关基因定义为保护性基因，M2 为风险基因，用于预测已发表的肺癌微阵列数据中的结果。与巨噬细胞共培养后，在体外和体内评估 A549 细胞的表型。结果：根据已发表的患者队列（n = 443）确定了三个基因特征，包括 M1 特异性基因特征、M2 特异性基因特征和 M1/M2 组合基因特征。具有高风险基因特征的患者表现出更短的中位数卵巢...