Assessment of bacterial exposure on phagocytic capability and surface marker expression of sputum macrophages and neutrophils in COPD patients,Clinical & Experimental Immunology

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Assessment of bacterial exposure on phagocytic capability and surface marker expression of sputum macrophages and neutrophils in COPD patients
Clinical & Experimental Immunology ( IF 3.4 ) Pub Date : 2021-06-18 , DOI: 10.1111/cei.13638
Simon Lea ₁ , Rosemary Gaskell ₁ , Simon Hall ₂ , Barbara Maschera ₂ , Edith Hessel ₃ , Dave Singh _{1,

4}

Affiliation

Defective phagocytosis has been shown in chronic obstructive pulmonary disease (COPD) bronchoalveolar lavage and blood monocyte-derived macrophages. Phagocytic capabilities of sputum macrophages and neutrophils in COPD are unknown. We investigated phagocytosis in these cells from COPD patients and controls. Phagocytosis of Streptococcus pneumoniae or fluorescently labelled non-typeable Haemophilus influenzae (NTHi) by sputum macrophages and neutrophils was determined by gentamycin protection assay (COPD; n = 5) or flow cytometry in 14 COPD patients, 8 healthy smokers (HS) and 9 healthy never-smokers (HNS). Sputum macrophages and neutrophils were differentiated by adherence for the gentamycin protection assay or receptor expression (CD206 and CD66b, respectively), by flow cytometry. The effects of NTHi on macrophage expression of CD206 and CD14 and neutrophil expression of CD16 were determined by flow cytometry. There was greater uptake of S. pneumoniae [~10-fold more colony-forming units (CFU)/ml] by sputum neutrophils compared to macrophages in COPD patients. Flow cytometry showed greater NTHi uptake by neutrophils compared to macrophages in COPD (67 versus 38%, respectively) and HS (61 versus 31%, respectively). NTHi uptake by macrophages was lower in HS (31%, p = 0.019) and COPD patients (38%, p = 0.069) compared to HNS (57%). NTHi uptake by neutrophils was similar between groups. NTHi exposure reduced CD206 and CD14 expression on macrophages and CD16 expression on neutrophils. Sputum neutrophils showed more phagocytic activity than macrophages. There was some evidence that bacterial phagocytosis was impaired in HS sputum macrophages, but no impairment of neutrophils was observed in HS or COPD patients. These results highlight the relative contributions of neutrophils and macrophages to bacterial clearance in COPD.

中文翻译：

细菌暴露对慢性阻塞性肺病患者痰巨噬细胞和中性粒细胞吞噬能力及表面标志物表达的评估

慢性阻塞性肺疾病 (COPD) 支气管肺泡灌洗液和血液单核细胞衍生的巨噬细胞中已显示出吞噬作用缺陷。COPD 中痰巨噬细胞和中性粒细胞的吞噬能力尚不清楚。我们研究了慢性阻塞性肺病患者和对照组这些细胞的吞噬作用。通过庆大霉素保护试验 (COPD; n = 5) 或流式细胞术测定14名慢性阻塞性肺病患者、8 名健康吸烟者 (HS) 和 9 名健康人的痰巨噬细胞和中性粒细胞对肺炎链球菌或荧光标记的不可分型流感嗜血杆菌 (NTHi) 的吞噬作用从不吸烟者（HNS）。通过流式细胞术通过庆大霉素保护测定或受体表达（分别为 CD206 和 CD66b）的粘附来区分痰巨噬细胞和中性粒细胞。通过流式细胞术测定NTHi对巨噬细胞CD206和CD14表达以及中性粒细胞CD16表达的影响。与 COPD 患者中的巨噬细胞相比，痰中性粒细胞对肺炎链球菌的摄取量[~10 倍以上的菌落形成单位 (CFU)/ml]。流式细胞术显示，与巨噬细胞相比，COPD（分别为 67 %和38%）和 HS（分别为 61 %和31%）中中性粒细胞对 NTHi 的摄取更高。与 HNS (57%) 相比， HS (31%, p = 0.019) 和 COPD 患者 (38%, p = 0.069)巨噬细胞对 NTHi 的摄取较低。中性粒细胞对 NTHi 的摄取在各组之间相似。NTHi 暴露降低了巨噬细胞上的 CD206 和 CD14 表达以及中性粒细胞上的 CD16 表达。痰中性粒细胞比巨噬细胞表现出更强的吞噬活性。有一些证据表明 HS 痰巨噬细胞的细菌吞噬作用受损，但在 HS 或 COPD 患者中未观察到中性粒细胞受损。这些结果强调了中性粒细胞和巨噬细胞对慢性阻塞性肺病细菌清除的相对贡献。

更新日期：2021-06-18

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