This study developed a facile strategy for assembling gelatin monolayers on Titanium (Ti) surface and enabled coordinated regulation of the surface chemical composition, morphology, wetting, and charged properties. The monolayer architecture was tuned by controlling the gelatin conformation with n-alkylsulphate and n-alkylsulphonate. The increased alkyl chain length of the surfactants contributed to the decrease of Gibbs free energy and the increase of binding energy in the gelatin-surfactant, which promoted the development of a β-sheet structural framework. The β-sheet structures induced the reduction of the layer thickness, disturbed the density stacking states of nanoparticles formed from the gelatin-surfactant complex on the monolayers, and improved the adjustability of exposure density of amino groups on the thin-layer surface, resulting in a further optimization of the Ti sheets surface. The gelatin monolayers retained excellent stability even after immersion in normal saline for 7 days or in an incubator at 40 °C for 15 days. MTT assay, cell clone formation assay and cell adhesion/migration assays all demonstrated an excellent cyto-compatibility of the Ti-gelatin monolayer. This facile approach holds significant promise for applying in preparation of blood-contacting implant materials, especially cardiovascular scaffolds.

该研究开发了一种在钛 (Ti) 表面组装明胶单层的简便策略，并能够协调调节表面化学成分、形态、润湿和带电特性。通过用正烷基硫酸盐和正烷基磺酸盐控制明胶构象来调整单层结构。表面活性剂烷基链长的增加导致吉布斯自由能的降低和明胶-表面活性剂结合能的增加，促进了β-折叠结构框架的发展。该β-片状结构导致层厚降低，扰乱了明胶-表面活性剂复合物在单层上形成的纳米粒子的密度堆积状态，提高了薄层表面氨基暴露密度的可调性，导致进一步的钛板表面的优化。即使在生理盐水中浸泡 7 天或在 40°C 的培养箱中浸泡 15 天后，明胶单层仍保持出色的稳定性。MTT 测定、细胞克隆形成测定和细胞粘附/迁移测定都证明了 Ti-明胶单层的优良细胞相容性。这种简便的方法在制备接触血液的植入材料，尤其是心血管支架方面具有重要的应用前景。