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Genetic identification and characterisation of some Turkish sheep
Small Ruminant Research ( IF 1.6 ) Pub Date : 2021-06-18 , DOI: 10.1016/j.smallrumres.2021.106455
Emre Alarslan , Nezih Ata , Onur Yilmaz , Yasemin Öner , Cüneyt Kaptan , Türker Savaş , Alper Yilmaz

Turkey’s biological diversity is considerable because of its biogeographical positions, therefore it is plentiful in terms of diversity in animal genetic resources. Genetic diversity is important in sustainable breeding and conservation programs in livestock. The aim of this study was to determine the genetic structure of Yalova genotype, Kıvırcık, Tahirova and Eşme sheep breed by using twenty microsatellite markers. A total of 223 Turkish native unrelated sheep (Yalova, Kıvırcık, Tahirova and Eşme) which were raised in sixteen flocks located in four different provinces (Kırklareli, Çanakkale, Yalova ve Uşak) were investigated. A total of 543 alleles were found. The number of loci showed deviations from Hardy-Weinberg equilibrium ranged from 11 (Kıvırcık) to 17 (Yalova). Although a total of 183 private alleles were detected, only 21 of them had allele frequency above 0.05. The number of observed alleles differed from 42 (for OarFCB20 and ILSTS5) to 20 (for OarCP34 and DRBP1). The highest Ne and PIC values (19.23 and 0.95, respectively) were also observed for ILSTS5 locus. While expected heterozygosity (He) ranged between 0.83−0.95, observed heterozygosity differed from 0.69−0.95. These results can be used for the initiation of registration studies for Yalova after combining phenotypic data evaluations.



中文翻译:

一些土耳其绵羊的遗传鉴定和表征

由于其生物地理位置,土耳其的生物多样性相当可观,因此在动物遗传资源的多样性方面非常丰富。遗传多样性在牲畜的可持续育种和保护计划中很重要。本研究的目的是通过使用 20 个微卫星标记确定 Yalova 基因型、Kıvırcık、Tahirova 和 Eşme 绵羊品种的遗传结构。总共对 223 只土耳其本土无关绵羊(Yalova、Kıvırcık、Tahirova 和 Eşme)进行了调查,这些绵羊在位于四个不同省份(Kırklareli、Çanakkale、Yalova ve Uşak)的 16 个羊群中饲养。共发现 543 个等位基因。位点的数量显示出与 Hardy-Weinberg 平衡的偏差范围从 11 (Kıvırcık) 到 17 (Yalova)。虽然共检测到 183 个私有等位基因,其中只有 21 个等位基因频率高于 0.05。观察到的等位基因数量从 42(对于 OarFCB20 和 ILSTS5)到 20(对于 OarCP34 和 DRBP1)不等。还观察到 ILSTS5 基因座的最高 Ne 和 PIC 值(分别为 19.23 和 0.95)。虽然预期杂合度 (He) 在 0.83-0.95 之间,但观察到的杂合度与 0.69-0.95 不同。在结合表型数据评估后,这些结果可用于启动 Yalova 的注册研究。

更新日期:2021-06-20
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