Mitochondrial genomes (mitogenomes) of metazoans typically contain 37 genes, comprising 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. To date, complete mitogenome sequences of 15 species of Astigmatina have been elucidated; and they contain variations in several features, such as gene order, unconventional tRNA secondary structures, and the number and internal structures of control regions. Here, we sequenced the mitogenomes of Lepidoglyphus destructor and Gohieria fusca, thus representing the first reported complete mitogenome sequence of mite species from the superfamily Glycyphagoidea. In total, 37 mitochondrial genes were identified in both species. The same gene arrangement pattern was found in both species, and this pattern was consistent with that in the possible common ancestor of astigmatid mites. Most tRNAs were highly truncated in both species, which is typical of acariform mites. The largest non-coding region in L. destructor and G. fusca can be divided into two domains based on AT content and stem-loop structures. Domain I has a stem-loop structure; domain II is more AT-rich than domain I and includes several conserved sequences that are also observed in Acaridae and Pyroglyphidae. The largest non-coding region of Acalvolia sp. (Astigmatina, Hemisarcoptoidea) also exhibits similar features. The phylogenetic relationships found in our study are consistent with those identified by previous phylogenetic studies on astigmatid mites. Within Astigmatina, Glycyphagoidea was recovered as a monophyletic group.

后生动物的线粒体基因组（有丝分裂基因组）通常包含 37 个基因，包括 13 个蛋白质编码基因、2 个 rRNA 基因和 22 个 tRNA 基因。迄今为止，已阐明了 15 种 Astigmatina 的完整有丝分裂基因组序列；它们包含几个特征的变化，例如基因顺序、非常规 tRNA 二级结构以及控制区域的数量和内部结构。在这里，我们对Lepidoglyphus destructor和Gohieria fusca的有丝分裂基因组进行了测序，因此代表了来自 Glycyphagoidea 总科的螨类物种的第一个报道的完整有丝分裂基因组序列。总共在两个物种中鉴定了 37 个线粒体基因。在两个物种中都发现了相同的基因排列模式，这种模式与散光螨可能的共同祖先中的模式一致。大多数 tRNA 在两个物种中都被高度截断，这是典型的螨类。L. destructor和G. fusca 中最大的非编码区可以根据 AT 含量和茎环结构分为两个域。域 I 具有茎环结构；结构域 II 比结构域 I 更富含 AT，并且包括几个在 Acaridae 和 Pyroglyphidae 中也观察到的保守序列。Acalvolia最大的非编码区sp. (Astigmatina, Hemisarcoptoidea) 也表现出类似的特征。在我们的研究中发现的系统发育关系与先前对散光螨的系统发育研究确定的系统发育关系一致。在 Astigmatina 中，Glycyphagoidea 作为一个单系群被回收。