Platinum nanoparticles (PtNPs) have been investigated for their antioxidant abilities in a range of biological and other applications. The ability to reduce off-target cold atmospheric plasma (CAP) cytotoxicity would be useful in Plasma Medicine; however, little has been published to date about the ability of PtNPs to reduce or inhibit the effects of CAP. Here we investigate whether PtNPs can protect against CAP-induced cytotoxicity in cancerous and non-cancerous cell lines. PtNPs were shown to dramatically reduce intracellular reactive species (RONS) production in U-251 MG cells. However, RONS generation was unaffected by PtNPs in medium without cells. PtNPs protect against CAP induced mitochondrial membrane depolarization, but not cell membrane permeabilization which is a CAP-induced RONS-independent event. PtNPs act as potent intracellular scavengers of reactive species and can protect against CAP induced cytotoxicity. PtNPs, showing no significant biocorrosion, may be useful as a catalytic antioxidant for healthy tissue and for protecting against CAP-induced tissue damage.

铂纳米粒子 (PtNPs) 在一系列生物和其他应用中的抗氧化能力已被研究。降低脱靶冷大气等离子体 (CAP) 细胞毒性的能力将在血浆医学中有用；然而，迄今为止，关于 PtNPs 减少或抑制 CAP 作用的能力的报道很少。在这里，我们研究 PtNPs 是否可以保护癌细胞和非癌细胞系中 CAP 诱导的细胞毒性。PtNPs 可显着降低 U-251 MG 细胞中的细胞内活性物质 (RONS) 的产生。然而，在没有细胞的培养基中，RONS 的产生不受 PtNPs 的影响。PtNPs 可防止 CAP 诱导的线粒体膜去极化，但不能防止细胞膜透化，后者是 CAP 诱导的 RNS 非依赖性事件。PtNPs 作为活性物质的有效细胞内清除剂，可以防止 CAP 诱导的细胞毒性。PtNPs 没有显着的生物腐蚀，可用作健康组织的催化抗氧化剂和防止 CAP 诱导的组织损伤。