We performed several experiments using three strains of Virgibacillus salexigens, namely, P2, NT N53, and C-20Mo^T (DSM 11483^T), which were isolated from completely different sources, in relation to bacteriocin production ability. Results of whole-genome sequencing analysis revealed that all strains have very similar sequences encoding class IId bacteriocin. Although a partial amino acid sequence of the purified bacteriocin produced by strain P2 isolated from fermented food was previously reported, whole-genome sequencing and the N-terminal sequencing results in this study showed that its complete amino acid sequence consisted of 48 residues, which corresponded to that of the hypothetical bacteriocin encoded by the gene in Virgibacillus massiliensis strain Vm-5^T (DSM 28587^T) isolated from the human gut. From the results of 16S rRNA gene sequencing and whole-genome sequencing analyses, we taxonomically confirmed Vm-5^T to be a strain of V. salexigens, and its broth culture showed antibacterial activity. Strain NT N53 isolated from the deep-sea floor produced two bacteriocins, namely, NTN-A and NTN-B. The results of N-terminal sequencing, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and whole-genome sequencing analyses showed that their amino acid sequences differed in only one residue, and NTN-A showed the same sequence as the bacteriocin produced by strain P2. Although strain C-20Mo^T isolated from a solar saltern had the coding sequence very similar to that of NTN-A, its broth culture showed no antibacterial activity. This finding suggests that class IId bacteriocin-producing or bacteriocin-gene-encoding V. salexigens strains are widely distributed in distinct environment sources with different geographical and material properties.

我们使用从完全不同的来源分离的三种Virgibacillus salexigens菌株，即 P2、NT N53 和 C-20Mo ^T (DSM 11483 ^T )进行了几个与细菌素生产能力相关的实验。全基因组测序分析的结果表明，所有菌株都具有非常相似的编码 IId 类细菌素的序列。虽然之前报道了从发酵食品中分离出的菌株 P2 产生的纯化细菌素的部分氨基酸序列，但本研究的全基因组测序和 N 端测序结果表明，其完整的氨基酸序列由 48 个残基组成，对应由基因编码的假设细菌素从人类肠道中分离出的Virgibacillus massiliensis菌株 Vm-5 ^T (DSM 28587 ^T )。从16S rRNA基因测序和全基因组测序分析结果，我们在分类学上证实Vm-5 ^T是一株V. salexigens，其肉汤培养物显示出抗菌活性。从深海海底分离的菌株NT N53产生两种细菌素，即NTN-A和NTN-B。N端测序、基质辅助激光解吸/电离飞行时间质谱和全基因组测序分析的结果表明，它们的氨基酸序列仅在一个残基上不同，NTN-A显示出相同的序列。菌株 P2 产生的细菌素。虽然应变 C-20Mo ^T从日光浴中分离出的编码序列与 NTN-A 的编码序列非常相似，其肉汤培养物没有显示出抗菌活性。这一发现表明，产生 IId 类细菌素或编码细菌素基因的V. salexigens菌株广泛分布在具有不同地理和材料特性的不同环境来源中。