The pentadentate ligand and the precursors were combined to form complexes by green approach. The ligand formation was confirmed by UV–Vis, FT-IR, ¹H-NMR, and LC-MS. The optimised stable structure was obtained by molecular simulation studies and the complexes were interpreted by conductivity measurements, UV–Vis, FT-IR, magnetic susceptibility, VSM, and ESR spectral studies. The redox nature of the complexes was investigated by cyclic voltammetry. The cyclic voltammogram shows complexes exhibited single electron transfer from Cu⁺²/Cu⁺¹. Complexes and penta-dentate ligand were screened for in vitro cytotoxicity by MTT assay method on A431 skin cancer cell line. The ligand structural stability and biological activity were confirmed by theoretical computational studies. The magnetic behaviour showed antiferromagnetic properties at low temperature. The complexes were used as high bar magnets. Similarly, the redox behaviour showed that the complexes could be used in electroplating techniques and sensors. Clinical application revealed that the complexes had effective cytotoxicity. From the data obtained, the complexes were in the form [MLR], where L was the penta-dentate ligand and R = [C₆H₅COO] & R = [C₆H₄COO (OH)].

五齿配体和前体通过绿色方法结合形成复合物。配体的形成通过 UV-Vis、FT-IR、¹ H-NMR 和 LC-MS 确认。通过分子模拟研究获得了优化的稳定结构，并通过电导率测量、UV-Vis、FT-IR、磁化率、VSM 和 ESR 光谱研究来解释复合物。通过循环伏安法研究了配合物的氧化还原性质。循环伏安图显示复合物表现出来自 Cu ⁺² /Cu ^{+1 的}单电子转移。体外筛选复合物和五齿配体MTT法测定A431皮肤癌细胞系的细胞毒性。通过理论计算研究证实了配体结构稳定性和生物活性。磁性在低温下表现出反铁磁性。复合物用作高条形磁铁。同样，氧化还原行为表明该配合物可用于电镀技术和传感器。临床应用表明该复合物具有有效的细胞毒性。根据获得的数据，复合物的形式为 [MLR]，其中 L 是五齿配体，R = [C ₆ H ₅ COO] & R = [C ₆ H ₄ COO (OH)]。