The exposure of school children to indoor air pollutants has increased allergy and respiratory diseases. The objective of this study were to determine the toxicodynamic interaction of indoor pollutants exposure, biological and chemical with expression of adhesion molecules on eosinophil and neutrophil. A self-administered questionnaire, allergy skin test, and fractional exhaled nitric oxide (FeNO) analyser were used to collect information on health status, sensitization to allergens and respiratory inflammation, respectively among school children at age of 14 years. The sputum induced were analysed to determine the expression of CD11b, CD35, CD63 and CD66b on eosinophil and neutrophil by using flow cytometry technique. The particulate matter (PM_2.5 and PM₁₀), NO₂, CO₂, and formaldehyde, temperature, and relative humidity were measured inside the classrooms. The fungal DNA were extracted from settled dust collected from classrooms and evaluated using metagenomic techniques. We applied chemometric and regression in statistical analysis. A total of 1869 unique of operational taxonomic units (OTUs) of fungi were identified with dominated at genus level by Aspergillus (15.8%), Verrucoconiothyrium (5.5%), and Ganoderma (4.6%). Chemometric and regression results revealed that relative abundance of T. asahii were associated with down regulation of CD66b expressed on eosinophil, and elevation of FeNO levels in predicting asthmatic children with model accuracy of 63.6%. Meanwhile, upregulation of CD11b expressed on eosinophil were associated with relative abundance of A. clavatus and regulated by PM_2.5. There were significant association of P. bandonii with upregulation of CD63 expressed on neutrophil and exposure to NO₂. Our findings indicate that exposure to PM_2.5, NO₂, T. asahii, P.bandonii and A.clavatus are likely interrelated with upregulation of activation and degranulation markers on both eosinophil and neutrophil.

学童接触室内空气污染物增加了过敏和呼吸道疾病。本研究的目的是确定室内污染物暴露、生物和化学的毒理学相互作用与粘附分子在嗜酸性粒细胞和中性粒细胞上的表达。使用自填式问卷、过敏性皮试和呼出气一氧化氮 (FeNO) 分析仪分别收集 14 岁学龄儿童的健康状况、对过敏原的敏感性和呼吸道炎症的信息。用流式细胞术分析诱导的痰液，测定嗜酸性粒细胞和中性粒细胞上CD11b、CD35、CD63和CD66b的表达。颗粒物（PM _2.5和 PM ₁₀），NO ₂测量了教室内的、CO ₂和甲醛、温度和相对湿度。从教室收集的沉降灰尘中提取真菌 DNA，并使用宏基因组技术进行评估。我们在统计分析中应用了化学计量学和回归。共有 1869 个独特的真菌操作分类单元 (OTU) 被确定在属水平上以曲霉属(15.8%)、疣状孢霉(5.5%) 和灵芝(4.6%) 为主。化学计量学和回归结果表明，T. asahii 的相对丰度与嗜酸性粒细胞 CD66b 表达下调和 FeNO 水平升高有关，预测哮喘儿童的模型准确率为 63.6%。同时，嗜酸性粒细胞上表达的 CD11b 的上调与A. clavatus 的相对丰度相关，并受 PM _2.5调节。P. bandonii与中性粒细胞上表达的 CD63 上调和暴露于 NO ₂有显着关联。我们的研究结果表明，暴露于 PM _2.5 , NO ₂ , T. asahii , P.bandonii和A.clavatus 可能与嗜酸性粒细胞和中性粒细胞的活化和脱颗粒标志物的上调相关。