Due to the toxicity of bioaccumulative organohalides to human beings and ecosystems, a variety of biotic and abiotic remediation methods have been developed to remove organohalides from contaminated environments. Bioremediation employing organohalide-respiring bacteria (OHRB)-mediated microbial reductive dehalogenation (Bio-RD) represents a cost-effective and environmentally friendly approach to attenuate highly-halogenated organohalides, specifically organohalides in soil, sediment and other anoxic environments. Nonetheless, many factors severely restrict the implications of OHRB-based bioremediation, including incomplete dehalogenation, low abundance of OHRB and consequent low dechlorination activity. Recently, the development of in situ chemical oxidation (ISCO) based on sulfate radicals (SO ^·−₄ ) via the persulfate activation and oxidation (PAO) process has attracted tremendous research interest for the remediation of lowly-halogenated organohalides due to its following advantages, e.g., complete attenuation, high reactivity and no selectivity to organohalides. Therefore, integration of OHRB-mediated Bio-RD and subsequent PAO (Bio-RD-PAO) may provide a promising solution to the remediation of organohalides. In this review, we first provide an overview of current progress in Bio-RD and PAO and compare their limitations and advantages. We then critically discuss the integration of Bio-RD and PAO (Bio-RD-PAO) for complete attenuation of organohalides and its prospects for future remediation applications. Overall, Bio-RD-PAO opens up opportunities for complete attenuation and consequent effective in situ remediation of persistent organohalide pollution.

由于生物累积性有机卤化物对人类和生态系统的毒性，已经开发了多种生物和非生物修复方法来从污染环境中去除有机卤化物。采用有机卤化物呼吸细菌 (OHRB) 介导的微生物还原脱卤 (Bio-RD) 的生物修复代表了一种经济高效且环境友好的方法，可以减轻高度卤化的有机卤化物，特别是土壤、沉积物和其他缺氧环境中的有机卤化物。尽管如此，许多因素严重限制了基于 OHRB 的生物修复的影响，包括脱卤不完全、OHRB 丰度低以及随之而来的低脱氯活性。最近，基于硫酸根（SO ^·- ₄ ) 通过过硫酸盐活化和氧化 (PAO) 过程，由于其以下优点，例如完全衰减、高反应性和对有机卤化物没有选择性，在低卤化有机卤化物的修复方面引起了极大的研究兴趣。因此，OHRB 介导的 Bio-RD 和随后的 PAO（Bio-RD-PAO）的整合可能为有机卤化物的修复提供有希望的解决方案。在这篇综述中，我们首先概述了 Bio-RD 和 PAO 的当前进展，并比较了它们的局限性和优势。然后，我们批判性地讨论了 Bio-RD 和 PAO（Bio-RD-PAO）的整合，以完全衰减有机卤化物及其未来修复应用的前景。全面的，