Although high-solid enzymatic hydrolysis has emerged as an efficient strategy of sugar production from lignocellulosic materials, this process requires several steps to increase its efficiency. Current strategies include treatments to remove noncellulosic macromolecules. Here, dilute alkaline extraction was controlled to remove loosely bound xylan from sugarcane bagasse, and 14.7% (w/w) was recovered by ethanol precipitation. To the supernatant, sodium sulfite (5% w/w) was added to pretreat the residual sugarcane bagasse with alkaline sulfite ethanol (ASE). The sequential process allowed the removal of 85% of lignin, producing a carbohydrate-enriched solid. Incubation of ASE-pretreated bagasse with xylanase released up to 43% of the xylan as oligomers. Subsequent enzymatic saccharification was performed at initial solid loadings, 5, 10, and 15%. The conversion of ASE-pretreated bagasse yielded 84.4% glucan hydrolysis or 75 g/L glucose at 15% solids after 48 h, while samples with reduced xylan contents had lower water retention, and achieved relevant glucan hydrolysis yield (73%) and glucose titer (87 g/L). Enzymatic saccharification at high solid loading was dependent on xylan removal, and control of excess residual xylan may play an important role in fine-tuning product formation in high-solid enzymatic hydrolysis of the glucan fraction.

尽管高固体酶水解已成为从木质纤维素材料生产糖的有效策略，但该过程需要几个步骤来提高其效率。目前的策略包括去除非纤维素大分子的治疗。在这里，控制稀碱提取以从甘蔗渣中去除松散结合的木聚糖，并通过乙醇沉淀回收 14.7% (w/w)。向上清液中加入亚硫酸钠 (5% w/w)，用碱性亚硫酸盐乙醇 (ASE) 预处理残留的甘蔗渣。连续过程允许去除 85% 的木质素，产生富含碳水化合物的固体。ASE 预处理的甘蔗渣与木聚糖酶的孵育释放了高达 43% 的木聚糖寡聚体。随后的酶糖化在初始固体负载量 5、10 和 15% 下进行。ASE 预处理甘蔗渣的转化率在 48 小时后产生了 84.4% 的葡聚糖水解率或 75 g/L 的 15% 固体葡萄糖，而木聚糖含量降低的样品保水率较低，并实现了相关的葡聚糖水解率 (73%) 和葡萄糖滴度(87 克/升)。高固体负载下的酶促糖化取决于木聚糖的去除，并且控制过量残留木聚糖可能在葡聚糖级分的高固体酶促水解中的微调产物形成中起重要作用。