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Measuring Plant Metabolite Abundance in Spearmint (Mentha spicata L.) with Raman Spectra to Determine Optimal Harvest Time
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-06-17 , DOI: 10.1021/acsfoodscitech.1c00047 Jingzhe Li 1 , Chamari S. Wijesooriya 2 , Sadie J. Burkhow 1 , Linda K. B. Brown 3 , Beatrice Y. Collet 3 , John A. Greaves 3 , Emily A. Smith 1
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-06-17 , DOI: 10.1021/acsfoodscitech.1c00047 Jingzhe Li 1 , Chamari S. Wijesooriya 2 , Sadie J. Burkhow 1 , Linda K. B. Brown 3 , Beatrice Y. Collet 3 , John A. Greaves 3 , Emily A. Smith 1
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A fast field-deployable method utilizing Raman spectroscopy to determine the optimal harvest time of plants to extract the highest abundance of target metabolites is presented. Rosmarinic acid is a metabolite extracted from spearmint (Mentha spicata L.). Leaves from commercial “Native” and proprietary clonal line “KI110” spearmint were measured as a function of cell type and age to determine rosmarinic acid abundance. A linear regression model with leave-one-out cross-validation (R2CV = 0.61, RMSECV = 11.1 mg/g) was developed between selected Raman peak areas and rosmarinic acid concentrations determined by high-performance liquid chromatography (HPLC). A principal component analysis (PCA) model was also developed to determine rosmarinic acid abundance. The method may be suited to the analysis of many agriculturally relevant plant species and metabolites with distinct Raman peaks.
中文翻译：
用拉曼光谱测量留兰香 ( Mentha spicata L.) 中的植物代谢物丰度以确定最佳收获时间
提出了一种利用拉曼光谱确定植物最佳收获时间以提取最高丰度目标代谢物的快速现场部署方法。迷迭香酸是从留兰香 ( Mentha spicata L.) 中提取的代谢物。作为细胞类型和年龄的函数测量来自商业“原生”和专有克隆系“KI110”留兰香的叶子，以确定迷迭香酸的丰度。具有留一法交叉验证的线性回归模型 ( R 2 CV= 0.61, RMSECV = 11.1 mg/g) 在选定的拉曼峰面积和通过高效液相色谱 (HPLC) 测定的迷迭香酸浓度之间产生。还开发了主成分分析 (PCA) 模型来确定迷迭香酸的丰度。该方法可能适用于分析许多具有不同拉曼峰的农业相关植物物种和代谢物。
更新日期：2021-07-16
中文翻译：
用拉曼光谱测量留兰香 ( Mentha spicata L.) 中的植物代谢物丰度以确定最佳收获时间
提出了一种利用拉曼光谱确定植物最佳收获时间以提取最高丰度目标代谢物的快速现场部署方法。迷迭香酸是从留兰香 ( Mentha spicata L.) 中提取的代谢物。作为细胞类型和年龄的函数测量来自商业“原生”和专有克隆系“KI110”留兰香的叶子，以确定迷迭香酸的丰度。具有留一法交叉验证的线性回归模型 ( R 2 CV= 0.61, RMSECV = 11.1 mg/g) 在选定的拉曼峰面积和通过高效液相色谱 (HPLC) 测定的迷迭香酸浓度之间产生。还开发了主成分分析 (PCA) 模型来确定迷迭香酸的丰度。该方法可能适用于分析许多具有不同拉曼峰的农业相关植物物种和代谢物。
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