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Crystal structures of Scone: pseudosymmetric folding of a symmetric designer protein
Acta Crystallographica Section D ( IF 2.6 ) Pub Date : 2021-06-18 , DOI: 10.1107/s2059798321005787 B Mylemans 1 , T Killian 1 , L Vandebroek 1 , L Van Meervelt 2 , J R H Tame 3 , T N Parac-Vogt 4 , A R D Voet 1
Acta Crystallographica Section D ( IF 2.6 ) Pub Date : 2021-06-18 , DOI: 10.1107/s2059798321005787 B Mylemans 1 , T Killian 1 , L Vandebroek 1 , L Van Meervelt 2 , J R H Tame 3 , T N Parac-Vogt 4 , A R D Voet 1
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Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric β-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives.
中文翻译：
Scone 的晶体结构：对称设计蛋白的假对称折叠
近年来，计算蛋白质的发展有所增加，包括对称蛋白质。最近开发了一种名为 Cake 的九重对称 β-螺旋桨蛋白。在这里，尝试使用计算设计将这种蛋白质进一步设计成三重对称的九叶螺旋桨。设计了两种九叶螺旋桨蛋白，命名为 Scone-E 和 Scone-R。然而，晶体学揭示了两种设计的结构都采用了带有扭曲末端的八重构象，从而产生了一种假对称蛋白质。其中一种蛋白质只能在添加多金属氧酸盐后结晶，突出了这些分子作为结晶添加剂的有用性。
更新日期：2021-07-02
中文翻译：
Scone 的晶体结构：对称设计蛋白的假对称折叠
近年来，计算蛋白质的发展有所增加，包括对称蛋白质。最近开发了一种名为 Cake 的九重对称 β-螺旋桨蛋白。在这里，尝试使用计算设计将这种蛋白质进一步设计成三重对称的九叶螺旋桨。设计了两种九叶螺旋桨蛋白，命名为 Scone-E 和 Scone-R。然而，晶体学揭示了两种设计的结构都采用了带有扭曲末端的八重构象，从而产生了一种假对称蛋白质。其中一种蛋白质只能在添加多金属氧酸盐后结晶，突出了这些分子作为结晶添加剂的有用性。
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