Equilibrium passive sampling employing polydimethylsiloxane (PDMS) as a sampling phase can be used for the extraction of complex mixtures of organic chemicals from lipid-rich biota. We extended the method to lean tissues and more hydrophilic chemicals by implementing a mass-balance model for partitioning between lipids, proteins, and water in tissues and by accelerating uptake kinetics with a custom-built stirrer that effectively decreased time to equilibrium to less than 8 days even for a homogenized liver tissue with an only 4% lipid content. The partition constants log K_lipid/PDMS between tissues and PDMS were derived from measured concentration in PDMS and the mass-balance model and were very similar for 40 neutral chemicals with octanol–water partition constants 1.4 < log K_ow < 8.7, that is, log K_lipid/PDMS of 1.26 (95% CI, 1.13–1.39) for the adipose tissue, 1.16 (1.00–1.33) for the liver, and 0.58 (0.42–0.73) for the brain. This conversion factor can be applied to interpret chemical analysis and in vitro bioassays after additionally accounting for a small fraction of coextracted lipids of <0.7% of the PDMS weight. PDMS is more widely applicable for passive sampling of mammalian tissues than previously thought, both, in terms of diversity of chemicals and the range of lipid contents of tissues and, therefore, an ideal method for human biomonitoring to be combined with in vitro bioassays.

中文翻译：

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不同哺乳动物组织中有机化学物质与聚合物的平衡被动采样动力学

采用聚二甲基硅氧烷 (PDMS) 作为采样相的平衡被动采样可用于从富含脂质的生物群中提取有机化学品的复杂混合物。我们通过实施质量平衡模型在组织中的脂质、蛋白质和水之间进行分配，并通过使用定制的搅拌器加速吸收动力学，将达到平衡的时间有效地减少到小于 8，将方法扩展到瘦组织和更亲水的化学物质即使对于脂质含量仅为 4% 的均质肝组织，也需要几天的时间。组织和_PDMS之间的分配常数 log K_{脂质 / PDMS}来自 PDMS 中的测量浓度和质量平衡模型，并且对于辛醇-水分配常数为 1.4 < log K 的40 种中性化学品非常相似_ow < 8.7，即脂肪组织的log K_脂质/PDMS为 1.26（95% CI，1.13-1.39），肝脏为 1.16（1.00-1.33），大脑为 0.58（0.42-0.73）。此转换因子可用于解释化学分析和体外生物测定，另外占 PDMS 重量 <0.7% 的一小部分共提取脂质。PDMS 比以前认为的更广泛地适用于哺乳动物组织的被动采样，无论是在化学物质的多样性方面还是在组织的脂质含量范围方面，都是将人体生物监测与体外生物测定相结合的理想方法。