Developing fit-to-purpose analytical screening methods for plant marker constituents by simple and affordable techniques represents a constant and important request from the current transformation chain of plant products. In this work, methods developed on classical thin-layer chromatography (TLC) and overpressured layer chromatography (OPLC) have been applied on some of the most valuable commercial plant products, such as star anise (Illicium verum Hook. f.) essential oil and extracts, common thyme (Thymus vulgaris L.) and sweet fennel (Foeniculum vulgare Mill.) essential oils and acerola fruit (Malpighia punicifolia L.) hydroalcoholic extracts, to evaluate OPLC potentiality in comparison with TLC performances in the detection of some characteristic constituents. OPLC provided higher performance with respect to TLC in all experiments performed due to higher selectivity demonstrated on all the tested marker compounds except for flavonoids in acerola extracts. Considering the usage of planar chromatography in the quality control of plant derivatives, the present paper shows how the OPLC protocols were both highly time- and solvent-saving in comparison with classical TLC.

通过简单且负担得起的技术开发适合用途的植物标记成分分析筛选方法代表了当前植物产品转化链的持续和重要要求。在这项工作中，在经典薄层色谱 (TLC) 和超压层色谱 (OPLC) 上开发的方法已应用于一些最有价值的商业植物产品，如八角 ( Illicium v​​erum Hook. f.) 精油和提取物、百里香 ( Thymus vulgaris L.) 和甜茴香 ( Foeniculum vulgare Mill.) 精油和针叶樱桃( Malpighia punicifolia)L.) 水醇提取物，与 TLC 在检测某些特征成分方面的性能相比，评估 OPLC 的潜力。由于对所有测试的标记化合物（针叶树提取物中的黄酮类化合物除外）均表现出更高的选择性，因此在进行的所有实验中，OPLC 提供了相对于 TLC 的更高性能。考虑到平面色谱在植物衍生物质量控制中的使用，本文展示了 OPLC 协议与经典 TLC 相比如何既节省时间又节省溶剂。