ABSTRACT

Asparagus (Asparagus officinalis) is a worldwide important vegetable. Assessing the genetic diversity and relationships in a germplasm collection is a key step in the design of breeding programmes. The aim of this work was to evaluate the genetic diversity in asparagus from a wide array of origins assessed by Sequence Related Amplified Polymorphism (SRAP) markers. Twenty-four accessions of A. officinalis from 13 different origins and one accession of A. pseudoscaber were considered. Sixteen combinations of SRAP primers were tested. A total of 329 bands were obtained (98.48% polymorphism). The average polymorphic information content was 0.20. Hierarchical clustering was carried out, the dendrogram was plotted with bootstrap p-values for the different branches and the Dice’s distance matrix was represented by a Principal Coordinates Analysis. It was not possible to establish a clear origin-related grouping. The reduced cycles of selection accomplished along with the contemporaneous plant breeding and the open-pollinated nature of the species, could have precluded a marked differentiation in the species related to origins or cultivated to wild status. The genetic base of asparagus is not as narrow as previously thought. The information of the genetic relationships among asparagus germplasm could be useful to delineate crosses in order to seek potential hybrid vigour.

摘要

芦笋（Asparagus officinalis）是一种世界性的重要蔬菜。评估种质资源库中的遗传多样性和关系是育种计划设计的关键步骤。这项工作的目的是通过序列相关扩增多态性 (SRAP) 标记评估来自广泛来源的芦笋的遗传多样性。考虑了来自 13 个不同来源的 24 个A. officinalis种质和A. pseudoscaber的一种种质。测试了 16 种 SRAP 引物组合。共获得329条带（多态性98.48%）。平均多态信息含量为0.20。进行了层次聚类，用 bootstrap p绘制了树状图-不同分支的值和骰子的距离矩阵由主坐标分析表示。不可能建立一个明确的与起源相关的分组。减少的选择周期与同期的植物育种和物种的开放授粉性质一起完成，可能已经排除了与起源或栽培到野生状态相关的物种的显着分化。芦笋的遗传基础并不像以前想象的那么狭窄。芦笋种质之间的遗传关系信息可用于描绘杂交以寻求潜在的杂种优势。