Mycogone perniciosa is a mycoparasite causing Wet Bubble Diseases (WBD) of Agaricus bisporus. In the present study, the whole genome of M. perniciosa strain MgR1 was sequenced using Illumina NextSeq500 platform. This sequencing generated 8.03 Gb of high-quality data and a draft genome of 39 Mb was obtained through a de novo assembly of the high-quality reads. The draft genome resulted into prediction of 9276 genes from the 1597 scaffolds. NCBI-based homology analysis revealed the identification of 8660 genes. Notably, non-redundant protein database analysis of the M. perniciosa strain MgR1 revealed its close relation with the Trichoderma arundinaceum. Moreover, ITS-based phylogenetic analysis showed the highest similarity of M. perniciosa strain MgR1 with Hypomyces perniciosus strain CBS 322.22 and Mycogone perniciosa strain PPRI 5784. Annotation of the 3917 genes of M. perniciosa strain MgR1 grouped in three major categories viz. biological process (2583 genes), cellular component (2013 genes), and molecular function (2919 genes). UniGene analysis identified 2967 unique genes in M. perniciosa strain MgR1. In addition, prediction of the secretory and pathogenicity-related genes based on the fungal database indicates that 1512 genes (16% of predicted genes) encode for secretory proteins. Moreover, out of 9276 genes, 1296 genes were identified as pathogenesis-related proteins matching with 51 fungal and bacterial genera. Overall, the key pathogenic genes such as lysine M protein domain genes, G protein, hydrophobins, and cytochrome P450 were also observed. The draft genome of MgR1 provides an understanding of pathogenesis of WBD in A. bisporus and could be utilized to develop novel management strategies.

Mycogone perniciosa是一种引起双孢蘑菇湿泡病 (WBD) 的真菌寄生虫。在本研究中，M的全基因组。使用 Illumina NextSeq500 平台对perniciosa菌株 MgR1 进行测序。该测序产生了 8.03 Gb 的高质量数据，并通过高质量读数的从头组装获得了 39 Mb 的基因组草图。基因组草图预测了来自 1597 个支架的 9276 个基因。基于 NCBI 的同源性分析揭示了 8660 个基因的鉴定。值得注意的是， M的非冗余蛋白质数据库分析。perniciosa菌株 MgR1 揭示了它与Trichoderma arundinaceum的密切关系。此外，基于 ITS 的系统发育分析显示M的相似性最高。perniciosa菌株 MgR1 与Hypomyces perniciosus菌株 CBS 322.22 和Mycogone perniciosa菌株 PPRI 5784。对M. perniciosa菌株 MgR1的 3917 个基因的注释分为三大类，即。生物过程（2583 个基因）、细胞成分（2013 个基因）和分子功能（2919 个基因）。UniGene 分析确定了M中的 2967 个独特基因。冻疮菌株 MgR1。此外，基于真菌数据库对分泌和致病性相关基因的预测表明，有 1512 个基因（占预测基因的 16%）编码分泌蛋白。此外，在9276个基因中，1296个基因被鉴定为与51个真菌和细菌属相匹配的发病相关蛋白。总体而言，还观察到了赖氨酸M蛋白结构域基因、G蛋白、疏水蛋白和细胞色素P450等关键致病基因。MgR1 的基因组草图提供了对双孢菌 WBD 发病机制的理解，可用于开发新的管理策略。