The current research focuses on the Intracellular biosynthesis of Ag/AgCl nanohybrids in microalgae, Scenedesmus sp. The effect of biosynthesis process on growth and lipid profile of cells is key element of this study. Ag/AgCl nanohybrids synthesized intracellularly were characterized by UV–Vis spectrophotometer, Powder X-Ray Diffraction (P-XRD), Scanning Electron Microscopy (SEM), High Resolution Transmission Electron Microscopy (HRTEM). 10–20 nm and 10–50 nm sized spherical shaped nanoparticles of polycrystalline nature were grown using 0.5 and 1 mM of AgNO₃ precursor, respectively and Scenedesmus sp. as reducing agent. Total lipid content of the cells treated with 0.5 mM and 1 mM AgNO₃ was static and found to be 43.2 ± 0.01 μg/mL and 48.2 ± 0.02 μg/mL respectively at 120 h of Ag/AgCl nanoparticles biosynthesis. FAME (Fatty Acid Methyl Ester) profile was improved due to intracellular nanoparticles biosynthesis with maximum C16:0 (palmitic acid) (35.7%) in cells treated with 0.5 mM AgNO₃ used for Ag/AgCl nanohybrids synthesis. Palmitic acid in cells exposed to 0.5 mM concentration of metallic precursor increased by 75.86%. Synthesized nanoparticles were tested on four bacterial strains to establish its antibacterial efficiency showing appropriate zone of inhibition at varying concentrations. Present study efficiently demonstrates the utility of microalgae integrating nanoparticles biosynthesis and lipid accumulation.

目前的研究重点是微藻中银/氯化银纳米杂化物的细胞内生物合成，栅藻属。生物合成过程对细胞生长和脂质谱的影响是本研究的关键要素。细胞内合成的 Ag/AgCl 纳米杂化物通过紫外-可见分光光度计、粉末 X 射线衍射 (P-XRD)、扫描电子显微镜 (SEM)、高分辨率透射电子显微镜 (HRTEM) 进行表征。分别使用 0.5 和 1 mM 的 AgNO ₃前体和栅栏藻生长了 10-20 nm 和 10-50 nm 大小的多晶球形纳米粒子。作为还原剂。用 0.5 mM 和 1 mM AgNO ₃处理的细胞的总脂质含量是静态的，在 Ag/AgCl 纳米颗粒生物合成 120 小时时发现分别为 43.2 ± 0.01 μg/mL 和 48.2 ± 0.02 μg/mL。FAME（脂肪酸甲酯）谱由于细胞内纳米颗粒生物合成而得到改善，在用 0.5 mM AgNO ₃处理的细胞中，C16:0（棕榈酸）（35.7%）最大，用于 Ag/AgCl 纳米杂化物合成。暴露于 0.5 mM 金属前体浓度的细胞中的棕榈酸增加了 75.86%。合成的纳米粒子在四种细菌菌株上进行了测试，以确定其抗菌效率，在不同浓度下显示出适当的抑菌圈。本研究有效地证明了微藻整合纳米颗粒生物合成和脂质积累的效用。