Anneslea fragrans Wall., commonly known as “Pangpo Tea”, is traditionally used as a folk medicine and healthy tea for the treatment of liver and intestine diseases. The aim of this study was to purify the antioxidative and cytoprotective polyphenols from A. fragrans leaves. After fractionation with polar and nonpolar organic solvents, the fractions of aqueous ethanol extract were evaluated for their total phenolic (TPC) and flavonoid contents (TFC) and antioxidant activities (DPPH, ABTS, and FRAP assays). The n-butanol fraction (BF) showed the highest TPC and TFC with the strongest antioxidant activity. The bio-guided chromatography of BF led to the purification of six flavonoids (1–6) and one benzoquinolethanoid (7). The structures of these compounds were determined by NMR and MS techniques. Compound 6 had the strongest antioxidant capacity, which was followed by 5 and 2. The protective effect of the isolated compounds on hydrogen peroxide (H₂O₂)-induced oxidative stress in HepG2 cells revealed that the compounds 5 and 6 exhibited better protective effects by inhibiting ROS productions, having no significant difference with vitamin C (p > 0.05), whereas 6 showed the best anti-apoptosis activity. The results suggest that A. fragrans could serve as a valuable antioxidant phytochemical source for developing functional food and health nutraceutical products.

中文翻译：

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从 Anneslea fragrans Wall 中分离酚类组合物的活性引导。及其对过氧化氢诱导的 HepG2 细胞氧化应激的细胞保护作用

Anneslea fragrans Wall.，俗称“庞坡茶”，传统上被用作治疗肝脏和肠道疾病的民间药物和保健茶。本研究的目的是从香椿叶中纯化抗氧化和细胞保护多酚。用极性和非极性有机溶剂分馏后，评估乙醇提取物的总酚 (TPC) 和类黄酮含量 (TFC) 以及抗氧化活性（DPPH、ABTS 和 FRAP 测定）。所述Ñ丁醇级分（BF）显示出最高的TPC和TFC具有最强的抗氧化活性。BF 的生物引导色谱法纯化了六种黄酮类化合物 ( 1 – 6 ) 和一种苯并喹乙醇 (7）。这些化合物的结构由NMR和MS技术确定。化合物6的抗氧化能力最强，其次是化合物5和化合物2。分离化合物对HepG2细胞过氧化氢（H ₂ O ₂）诱导的氧化应激的保护作用表明，化合物5和6通过抑制ROS产生表现出更好的保护作用，与维生素C无显着差异（p > 0.05 )，而6显示出最好的抗细胞凋亡活性。结果表明A. fragrans 可以作为一种有价值的抗氧化植物化学来源，用于开发功能性食品和健康营养产品。