The content of free and hydrolyzed primary amino acids was determined for five species of microalgae: Haematococcus pluvialis, Skeletonema costatum, Arthrospira sp., Acutodesmus acuminatus, and Botryococcus braunii. The amino acid profile was determined using an automated HPLC-DAD reverse phase pre-column analysis method, which is described in detail. Acid hydrolysis using 6 M HCl and autoclaving at 120 °C for 2 h was validated using casein of bovine origin as standard. Nitrogen to protein conversion factors were calculated for each microalgae. Haematococcus pluvialis contains the least amount of free amino acids (38.8 ± 0.15 mg AA/100 g DW), while Arthrospira sp. shows the most enriched free amino acid profile (316.1 ± 0,11 mg AA/100 g DW). Skeletonema costatum shows the least amount of amino acids after hydrolysis with 5.09 ± 0.28 g AA/100 g DW while Arthrospira sp. achieves 27.76 ± 0.19 g AA/100 g DW. The total protein content and its relationship with the detected amino acid content are discussed.

测定了五种微藻的游离和水解一级氨基酸的含量：雨生红球藻、肋骨骨架、节旋藻属。、Acutodesmus acuminatus和Botryococcus braunii 。氨基酸谱是使用自动 HPLC-DAD 反相柱前分析方法确定的，详细描述了该方法。使用 6 M HCl 酸水解并在 120 °C 下高压灭菌 2 小时，使用牛源酪蛋白作为标准进行验证。计算每种微藻的氮到蛋白质的转化因子。雨生红球藻含有最少的游离氨基酸 (38.8 ± 0.15 mg AA/100 g DW)，而节旋藻sp. 显示了最丰富的游离氨基酸谱 (316.1 ± 0.11 mg AA/100 g DW)。Skeletonema costatum水解后的氨基酸含量最少，为 5.09 ± 0.28 g AA/100 g DW，而节旋藻属。达到 27.76 ± 0.19 g AA/100 g DW。讨论了总蛋白质含量及其与检测氨基酸含量的关系。