ABSTRACT

OBJECTIVE: Although multiple sclerosis (MS) is known to be an immune-mediated disease, very little is known about its etiopathogenesis. MicroRNAs (miRNAs) are small non-coding proteins involved in the regulation of gene expression. T-cell activation potential in neurodegenerative diseases has been a research topic of interest in recent years Cytokines play an important role in the course and pathogenesis of MS, The aim of the present study was to analyze expression levels of miR-20, miR-21, miR-26, miR-155, and Let-7, which target the cytokines interleukin IL-17 and IL-23, in order to evaluate the relationship between MS and miRNAs that modulate the expression of cytokines involved in the autoimmune pathway.

MATERIALS and METHODS: The study included 20 relapsing-remitting multiple sclerosis (MS) patients who were at least 18 years of age and were undergoing outpatient immunomodulatory therapy and 20 healthy, unrelated individuals who had no systemic disease and were not taking any medication as a control group. Peripheral blood samples were collected from all participants into EDTA-containing tubes and plasma was isolated for cDNA synthesis. From these cDNA samples, miRNA expression levels were quantitatively analyzed via melting curve analysis using the miScript SYBR Green kit in a Rotor-Gene Q real-time PCR device.

RESULTS: Comparison of miRNA expression levels in the peripheral blood samples and MS patients and healthy subjects revealed that the MS patients had significant upregulation of miR-20 and downregulation of miR-26 and miR-155 compared to the control group (p<0.005).

CONCLUSION: Dysregulation of miRNA expression may play a role in the pathogenesis of MS.

摘要

目的：虽然已知多发性硬化症 (MS) 是一种免疫介导的疾病，但对其发病机制知之甚少。MicroRNAs (miRNAs) 是参与基因表达调控的小型非编码蛋白。神经退行性疾病中的 T 细胞活化潜能是近年来人们感兴趣的研究课题 细胞因子在 MS 的病程和发病机制中发挥着重要作用，本研究的目的是分析 miR-20、miR-21 的表达水平、miR-26、miR-155 和 Let-7，它们靶向细胞因子白细胞介素 IL-17 和 IL-23，以评估 MS 与调节参与自身免疫途径的细胞因子表达的 miRNA 之间的关系。

材料和方法：该研究包括 20 名至少 18 岁且正在接受门诊免疫调节治疗的复发缓解型多发性硬化症 (MS) 患者和 20 名没有全身性疾病且未服用任何药物的健康无关个体。控制组。将所有参与者的外周血样本收集到含有 EDTA 的试管中，并分离血浆用于 cDNA 合成。从这些 cDNA 样品中，miRNA 表达水平通过熔解曲线分析在 Rotor-Gene Q 实时 PCR 装置中使用 miScript SYBR Green 试剂盒进行定量分析。

结果：外周血样本与 MS 患者和健康受试者 miRNA 表达水平的比较显示，与对照组相比，MS 患者的 miR-20 显着上调，miR-26 和 miR-155 下调（p<0.005） .

结论：miRNA表达失调可能在MS的发病机制中起作用。