Abstract

Essential oils from the leaves and stems of Tinomiscium petiolare Hook.f. & Thomson were obtained by hydrodistillation and the chemical components were determined by gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC/MS). Also, the microdilution broth assay was used to test the antimicrobial activity of essential oils. The respective yields of the T. petiolare leaf and stem essential oils were 0.06 % and 0.04 % (v/w), calculated on a dry weight basis. The major components (percentages, respectively) identified from the leaf and stem essential oils of T. petiolare were relatively similar, comprising of β-bisabolene (11.43 % and 13.80 %), trans-α-bergamotene (6.52 % and 6.61 %), geranyl acetate (6.12 % and 5.77 %), (E)-caryophyllene (5.04 % and 4.73 %), δ-cadinene (4.10 % and 5.27 %), (E,E)-α-farnesene (4.54 % and 4.65 %) and α-zingiberene (3.85 % and 4.50 %). The antimicrobial activity test indicated that T. petiolare stem essential oil displayed moderate activity against Staphylococcus aureus ATCC 6538 with Minimum inhibitory concentration (MIC) value of 200 μg/mL, while both essential oils inhibited the growth of Pseudomonas aeruginosa ATCC 9027 with MIC value of 200 μg/mL. The chemical components and antimicrobial activity of the T. petiolare leaf and stem oils are reported for the first time.

摘要

来自Tinomiscium petiolare Hook.f的叶子和茎的精油。& Thomson 是通过加氢蒸馏得到的，化学成分通过气相色谱（GC）和气相色谱-质谱联用（GC/MS）测定。此外，微量稀释肉汤测定用于测试精油的抗菌活性。按干重计算，T. petiolare叶和茎精油的产量分别为 0.06 % 和 0.04 % (v/w)。从T. petiolare的叶和茎精油中鉴定出的主要成分（分别为百分比）相对相似，包括 β-红没药烯（11.43 % 和 13.80 %）、反式-α-佛手柑（6.52 % 和 6.61 %）、乙酸香叶酯（6.12 % 和 5.77 %）、（E）-石竹烯（5.04 % 和 4.73 %）、δ-卡二烯（4.10 % 和 5.27 %）、（E） )-α-法呢烯（4.54 % 和 4.65 %）和 α-姜烯（3.85 % 和 4.50 %）。抑菌活性测试表明，叶柄茎精油对金黄色葡萄球菌ATCC 6538表现出中等活性，最低抑菌浓度 (MIC) 值为 200 μg/mL，而两种精油均抑制铜绿假单胞菌ATCC 9027的生长，MIC 值为200 微克/毫升。首次报道了T. petiolare叶和茎油的化学成分和抗菌活性。