In this study, soils spiked with copper oxide nanoparticles (CuO NPs) or Cu(NO₃)₂ and aged as long as 90 days were utilized to investigate effect of soil properties and aging on oral and inhalation bioaccessibility of CuO NPs. Results showed that oral bioaccessibility of CuO NPs in gastric phase (GP) ranged from 70% to 84%, it significantly decreased to 50%–70% in intestinal phase (IP). The inhalation bioaccessibility of CuO NPs in artificial lysosomal fluid (ALF) ranged from 66% to 85%, and much higher than that in Gamble’s solution (GS, 3.3%–23%). By comparing CuO NPs to Cu(NO₃)₂ bioaccessibility, insignificant difference was found. The aging time (D15 and D90) had limited effect on their oral and inhalation bioaccessibility. CEC and free Al were positively and clay content was negatively correlated with CuO NPs inhalation bioaccessibility, while Cu(NO₃)₂ inhalation bioaccessibility decreased with increasing soil clay content and pH. Our findings provide an essential basis to evaluate the human health risks of CuO NPs.

在这项研究中，掺入氧化铜纳米颗粒 (CuO NPs) 或 Cu(NO ₃ ) ₂并老化长达 90 天的土壤被用来研究土壤性质和老化对 CuO NPs 的口服和吸入生物可及性的影响。结果表明，CuO NPs 在胃相 (GP) 的口服生物可及性范围为 70% 至 84%，在肠相 (IP) 中显着降低至 50% 至 70%。CuO NPs 在人工溶酶体液 (ALF) 中的吸入生物可及性范围为 66% 至 85%，远高于 Gamble 溶液中的吸入生物可及性 (GS, 3.3%–23%)。通过比较 CuO NPs 与 Cu(NO ₃ ) ₂生物可及性，发现差异不显着。老化时间（D15 和 D90）对其口服和吸入生物可及性的影响有限。CEC和游离Al呈正相关，粘土含量与CuO NPs吸入生物可及性呈负相关，而Cu(NO ₃ ) ₂吸入生物可接近性随着土壤粘土含量和pH值的增加而降低。我们的研究结果为评估 CuO NPs 的人类健康风险提供了重要基础。