Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.

活体显微镜 (IVM) 是一种强大的技术，能够以（亚）细胞分辨率对活体动物的内部组织进行成像。在这里，我们展示了一种基于硅胶的成像窗口，由完全灵活的无缝设计组成，非常适合生长组织和肿瘤的长期纵向 IVM。至关重要的是，我们展示了这个窗口，无需任何定制，适用于使用快速和标准化植入程序的小鼠的众多解剖位置。这种低成本设备代表了重大的技术和性能进步，有助于在高等生物的不同背景下进行活体成像，为软和脆弱组织的体内成像开辟了以前无法实现的途径。