The goal of the present work was to fabricate a new low-cost, easy-to-prepare, dual-channel fluorescence chemosensor comprised of acridine-diphenylacetyl moieties (NDA) to enable remarkable Sn⁴⁺ detection in water and biological medium. The resulting NDA–Sn⁴⁺ complex was utilized for the distinguished identification of Cr₂O₇^2- ions from other anions and biomolecules. These investigations involve the absorption, fluorescence, and electrochemical methods for the detection of Sn⁴⁺ and Cr₂O₇^2- ions in pure water. The mechanism for NDA-mediated Sn⁴⁺ detection was experimentally determined by FT-IR, NMR titrations, mass (ESI) analyses, and DFT calculations. The obtained results indicate that the NDA chemosensor possessed excellent performance characteristics including good water solubility and compatibility, quick response time (less than 10 s), high sensitivity (Sn⁴⁺ = 0.268 μM and Cr₂O₇^2- = 0.160 μM), and selectivity against coexisting metals, anions, amino acids, and peptides. The chemosensor NDA induced negligible toxicity in live cells and was successfully utilized as a biomarker for the tracking of Sn⁴⁺ in human normal and cancer cells. More importantly, NDA demonstrates distinguished recognition of Sn⁴⁺ in human cancer cells rather than in normal live cells. Additionally, NDA was shown to act as a mitochondria-targeted probe in FaDu cells.

目前工作的目标是制造一种新的低成本、易于制备、双通道荧光化学传感器，由吖啶-二苯乙酰基部分 ( NDA ) 组成，以实现水和生物介质中显着的 Sn ⁴⁺检测。所得的NDA –Sn ⁴⁺复合物用于从其他阴离子和生物分子中区分 Cr ₂ O ₇ ^2-离子。这些研究涉及用于检测纯水中 Sn ⁴⁺和 Cr ₂ O ₇ ^2-离子的吸收、荧光和电化学方法。NDA介导的Sn的机制⁴⁺检测通过 FT-IR、NMR 滴定、质量 (ESI) 分析和 DFT 计算通过实验确定。结果表明，NDA化学传感器具有良好的水溶性和相容性、响应时间快（小于10 s）、灵敏度高（Sn ⁴⁺ = 0.268 μM和Cr ₂ O ₇ ^2- = 0.160 μM）等优异性能特点，以及对共存金属、阴离子、氨基酸和肽的选择性。化学传感器NDA在活细胞中诱导的毒性可忽略不计，并成功用作跟踪人类正常细胞和癌细胞中 Sn ⁴⁺的生物标志物。更重要的是，保密协议证明了对人类癌细胞而不是正常活细胞中的 Sn ^{4+ 的}显着识别。此外，NDA被证明在 FaDu 细胞中充当线粒体靶向探针。