Generation of Yeast Protoplasts by Lytic Actions of Iron Oxide Magnetic Nanoparticles,Industrial & Engineering Chemistry Research

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Generation of Yeast Protoplasts by Lytic Actions of Iron Oxide Magnetic Nanoparticles
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2021-06-16 , DOI: 10.1021/acs.iecr.1c01816
Menglin Xiao ₁ , Niuniu Chen ₁ , Chengzhi He ₂ , Shuobo Shi ₂ , Qinfu Lu ₁ , Shanshan Lv ₁

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Preparation of protoplasts of Saccharomyces cerevisiae and Pichia pastoris was achieved by using iron oxide (Fe₃O₄) magnetic nanoparticles (MNPs). The protoplasts were characterized by optical microscopy, atomic force microscopy, scanning electron microscopy, and energy-dispersive spectroscopy, revealing cell wall breakage. In addition, the as-prepared protoplasts allowed regeneration, DNA extraction, and transformation. These results support the conviction that Fe₃O₄ MNPs exhibit an intrinsic yeast lytic activity. The demonstration of protoplast generation can be useful in providing a feasible platform for genetic manipulation of yeasts and opens doors for yeast-based biotechnological applications. Moreover, it is anticipated that, with appropriate modifications, this approach can be extended to a range of microbiomes of industrial importance.

中文翻译：

通过氧化铁磁性纳米颗粒的裂解作用产生酵母原生质体

使用氧化铁 (Fe ₃ O ₄ ) 磁性纳米粒子 (MNP)制备了酿酒酵母和毕赤酵母的原生质体。通过光学显微镜、原子力显微镜、扫描电子显微镜和能量色散光谱对原生质体进行表征，揭示细胞壁破裂。此外，所制备的原生质体允许再生、DNA 提取和转化。这些结果支持 Fe ₃ O ₄MNP 表现出内在的酵母裂解活性。原生质体生成的示范可用于为酵母基因操作提供可行的平台，并为基于酵母的生物技术应用打开大门。此外，预计通过适当的修改，这种方法可以扩展到一系列具有工业重要性的微生物组。

更新日期：2021-06-30

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