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Lupane-type triterpenoids from Acacia dealbata bark extracted by different methods
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2021-06-16 , DOI: 10.1016/j.indcrop.2021.113734
Vítor H. Rodrigues , Marcelo M.R. de Melo , Inês Portugal , Carlos M. Silva

The management of invasive species generates large amounts of biomass which end up in low value applications. However, these residues can potentially contain compounds with important bioactive properties. In this study the bark of Acacia dealbata Link (Fabaceae) was extracted by different methods targeting triterpenoids content and bulk product. Batch solid-liquid extraction (SLE-batch), Soxhlet extraction (n-hexane, dichloromethane, ethyl acetate and ethanol) and supercritical fluid extraction (SFE) (CO2 with/without ethanol or ethyl acetate) were performed under different pressure and/or temperature conditions in order to assess total extraction yield (ηTotal), extract concentrations and yields of lupenyl acetate and lupenone. The extracts composition was analysed by gas chromatography coupled to mass spectroscopy. Summarily: (i) the highest values of ηTotal were achieved with ethanol by both Soxhlet and SLE-batch, and by SFE at 300 bar, 80 ⁰C with 5 wt.% ethanol; (ii) the highest concentrations of triterpenoids were obtained with n-hexane by Soxhlet and SLE-batch and, and by SFE at 200 bar, 40 °C with 5 wt.% ethyl acetate; (iii) the maximum individual yields were achieved with ethanol by both Soxhlet and SLE-batch, and by SFE at 200 bar, 40 °C with 5 wt.% ethyl acetate. The solubilities of lupenyl acetate, lupenone, tetracosanoic acid and hexacosan-1-ol in the various solvents were theoretically estimated, providing important insights for the interpretation of the experimental results.



中文翻译:

不同方法提取的金合欢树皮羽扇豆型三萜类化合物

入侵物种的管理产生了大量的生物量,这些生物量最终用于低价值应用。然而,这些残留物可能含有具有重要生物活性特性的化合物。在这项研究中,针对三萜含量和散装产品,通过不同方法提取了Acacia dealbata Link(豆科)的树皮。批次固-液萃取(SLE分批),索氏提取(Ñ己烷,二氯甲烷,乙酸乙酯和乙醇)和超临界流体萃取(SFE)(CO 2有/无乙醇或乙酸乙酯)不同的压力和下进行/或温度条件以评估总提取率(η一种),提取物浓度和乙酸羽扇豆酯和羽扇豆酮的产量。通过与质谱联用的气相色谱分析提取物组成。概括地说:(i) 的最高值η一种分别通过索氏和 SLE 批次使用乙醇实现,并通过 SFE 在 300 bar、80 ⁰C 下使用 5 wt.% 乙醇实现;(ⅱ)用得到的三萜类化合物的浓度最高Ñ在索氏-己烷和SLE分批和,并且通过SFE 200巴,40℃,5%(重量)乙酸乙酯。(iii) Soxhlet 和 SLE-batch 和 SFE 在 200 bar、40 °C 和 5 wt.% 乙酸乙酯的情况下使用乙醇实现了最大的单个产率。理论上估计了乙酸羽扇豆酯、羽扇豆酮、二十四烷酸和六十二烷-1-醇在各种溶剂中的溶解度,为解释实验结果提供了重要的见解。

更新日期:2021-06-17
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