Comparative transient expression analyses on two conserved effectors of Colletotrichum orbiculare reveal their distinct cell death-inducing activities between Nicotiana benthamiana and melon,Molecular Plant Pathology

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Comparative transient expression analyses on two conserved effectors of Colletotrichum orbiculare reveal their distinct cell death-inducing activities between Nicotiana benthamiana and melon
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-06-16 , DOI: 10.1111/mpp.13078
Jinlian Chen ₁ , Yoshihiro Inoue ₁ , Naoyoshi Kumakura ₂ , Kazuyuki Mise ₁ , Ken Shirasu ₂ , Yoshitaka Takano ₁

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Colletotrichum orbiculare infects cucurbits, such as cucumber and melon (Cucumis melo), as well as the model Solanaceae plant Nicotiana benthamiana, by secreting an arsenal of effectors that suppress the immunity of these distinct plants. Two conserved effectors of C. orbiculare, called NLP1 and NIS1, induce cell death responses in N. benthamiana, but it is unclear whether they exhibit the same activity in Cucurbitaceae plants. In this study, we established a new Agrobacterium-mediated transient expression system to investigate the cell death-inducing activity of NLP1 and NIS1 in melon. NLP1 strongly induced cell death in melon but, in contrast to the effects seen in N. benthamiana, mutations either in the heptapeptide motif or in the putative glycosylinositol phosphorylceramide-binding site did not cancel its cell death-inducing activity in melon. Furthermore, NLP1 lacking the signal peptide caused cell death in melon but not in N. benthamiana. Study of the transient expression of NIS1 also revealed that, unlike in N. benthamiana, NIS1 did not induce cell death in melon. In contrast, NIS1 suppressed flg22-induced reactive oxygen species generation in melon, as seen in N. benthamiana. These findings indicate distinct cell death-inducing activities of NLP1 and NIS1 in these two plant species that C. orbiculare infects.

中文翻译：

对圆形炭疽病两个保守效应子的比较瞬时表达分析揭示了烟草和甜瓜之间独特的细胞死亡诱导活性

圆叶炭疽菌通过分泌一系列抑制这些独特植物免疫力的效应子来感染葫芦科植物，例如黄瓜和甜瓜 ( Cucumis melo )，以及茄科植物模型烟草(Nicotiana Benthamiana )。 C的两个保守效应子。 Orbulare称为 NLP1 和 NIS1，可诱导N细胞死亡反应。 Benthamiana ，但尚不清楚它们是否在葫芦科植物中表现出相同的活性。在本研究中，我们建立了一个新的农杆菌介导的瞬时表达系统来研究NLP1和NIS1在甜瓜中的细胞死亡诱导活性。 NLP1 强烈诱导甜瓜细胞死亡，但与N中观察到的效果相反。 Benthamiana中，七肽基序或假定的糖基肌醇磷酸神经酰胺结合位点的突变并没有取消其在甜瓜中诱导细胞死亡的活性。此外，缺乏信号肽的 NLP1 会导致甜瓜细胞死亡，但不会导致甜瓜细胞死亡。本塞米阿纳。 NIS1 瞬时表达的研究还表明，与N . Benthamiana 、NIS1 不会诱导甜瓜细胞死亡。相反，NIS1 抑制了 flg22 诱导的甜瓜中活性氧的产生，如N中所示。本塞米阿纳。这些发现表明 NLP1 和 NIS1 在这两种植物中具有不同的细胞死亡诱导活性， C .轮匝感染。

更新日期：2021-07-22

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