The genus Notiophilus Duméril, 1806 is a distinctive taxon of small, diurnal and morphologically similar beetles exhibiting large eyes and widened second elytral intervals. In this study we analysed the effectiveness of DNA barcodes to discriminate 67 specimens that represent 8 species of Notiophilus from Central Europe. Interspecific K2P distances below 2.2% were found for N. biguttatus (Fabricius, 1779) and N. quadripunctatus Dejean, 1826, whereas intraspecific distances with values > 2.2% were revealed for N. rufipes Curtis, 1829. An additional phylogenetic analysis of all available species revealed a close relationship of N. directus Casey, 1920, N. semistriatus Say, 1823, N. simulator Fall, 1906 and N. sylvaticus Dejean, 1831, possibly indicating a radiation of these species in North America. Low support values of most other nodes, however, do not allow additional phylogenetic conclusions.

中文翻译：

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关于 Notiophilus Duméril，1806（鞘翅目，Carabidae）：使用 DNA 条形码进行物种描述和系统发育

Notiophilus Duméril 属，1806 年是一种独特的分类群，属于小型、昼行性和形态相似的甲虫，表现出大眼睛和加宽的第二鞘翅间隔。在这项研究中，我们分析了 DNA 条形码区分 67 个样本的有效性，这些样本代表了来自中欧的 8 种 Notiophilus。N. biguttatus (Fabricius, 1779) 和 N. quadripunctatus Dejean, 1826 的种间 K2P 距离低于 2.2%，而 N. rufipes Curtis, 1829 的种内距离值> 2.2%。物种揭示了 N. directus Casey, 1920, N. semistriatus Say, 1823, N.simulator Fall, 1906 和 N. sylvaticus Dejean, 1831, 可能表明这些物种在北美有辐射。大多数其他节点的支持值较低，但是，