Stimulation and electroporation by nanosecond electric pulses (nsEP) are distinguished by a phenomenon of bipolar cancellation, which stands for a reduced efficiency of bipolar pulses compared to unipolar ones. When two pairs of stimulating electrodes are arrayed in a quadrupole, bipolar cancellation inhibits nsEP effects near the electrodes, where the electric field is the strongest. Two properly shaped and synchronized bipolar nsEP overlay into a unipolar pulse towards the center of the electrode array, thus canceling the bipolar cancellation (a “CANCAN effect”). High efficiency of the re-created unipolar nsEP outweighs the weakening of the electric field with distance and focuses nsEP effects to the center. In monolayers of CHO, BPAE, and HEK cells, CANCAN effect achieved by the interference of two bipolar nsEP enhanced electroporation up to tenfold, with a peak at the quadrupole center. Introducing a time interval between bipolar nsEP prevented the formation of a unipolar pulse and eliminated the CANCAN effect. Strong electroporation by CANCAN stimuli killed cells over the entire area encompassed by the electrodes, whereas the time-separated pulses caused ablation only in the strongest electric field near the electrodes. The CANCAN approach is promising for uniform tumor ablation and stimulation targeting away from electrodes.

纳秒电脉冲 (nsEP) 的刺激和电穿孔的特点是双极消除现象，这意味着与单极脉冲相比，双极脉冲的效率降低。当两对刺激电极排列在四极杆中时，双极消除会抑制电极附近的 nsEP 效应，那里的电场最强。两个形状适当且同步的双极 nsEP 叠加成朝向电极阵列中心的单极脉冲，从而消除双极消除（“CANCAN 效应”）。重新创建的单极 nsEP 的高效率超过了电场随距离的减弱，并将 nsEP 效应集中到中心。在 CHO、BPAE 和 HEK 细胞的单层中，通过两个双极 nsEP 的干扰实现的 CANCAN 效应将电穿孔增强至十倍，峰值位于四极杆中心。在双极 nsEP 之间引入时间间隔可防止形成单极脉冲并消除 CANCAN 效应。CANCAN 刺激的强电穿孔杀死了电极包围的整个区域的细胞，而时间分离的脉冲仅在电极附近最强的电场中引起消融。CANCAN 方法有望实现均匀的肿瘤消融和远离电极的刺激靶向。CANCAN 刺激的强电穿孔杀死了电极包围的整个区域的细胞，而时间分离的脉冲仅在电极附近最强的电场中引起消融。CANCAN 方法有望实现均匀的肿瘤消融和远离电极的刺激靶向。CANCAN 刺激的强电穿孔杀死了电极包围的整个区域的细胞，而时间分离的脉冲仅在电极附近最强的电场中引起消融。CANCAN 方法有望实现均匀的肿瘤消融和远离电极的刺激靶向。