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Microorganisms that are critical for the fermentation quality of paper mulberry silage
Food and Energy Security ( IF 4.0 ) Pub Date : 2021-06-15 , DOI: 10.1002/fes3.304 Linna Guo 1 , Xuekai Wang 1 , Yanli Lin 2 , Xueping Yang 1 , Kuikui Ni 1 , Fuyu Yang 1
Food and Energy Security ( IF 4.0 ) Pub Date : 2021-06-15 , DOI: 10.1002/fes3.304 Linna Guo 1 , Xuekai Wang 1 , Yanli Lin 2 , Xueping Yang 1 , Kuikui Ni 1 , Fuyu Yang 1
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Silage quality remains an important issue in farming, as do limitations in the range of products suitable for animal fodder. We therefore explored the microorganisms that are critical for the fermentation quality of paper mulberry silage. Low (unwilted) and high (wilted) dry matter (DM) paper mulberry were harvested at two cutting times. These were ensiled for 0, 3, 7, 14, and 56 days, respectively. Compared with unwilted silages, wilting significantly decreased (p < 0.05) silage pH value, ammonia-N concentration, and yeast counts but increased (p < 0.05) lactic acid content. In addition, higher (p < 0.05) crude protein (CP) contents were also observed in wilted silages. Next-generation sequencing (NGS) analysis revealed that wilting reduced the abundance of Enterobacter, while increasing that of Lactobacillus. Single-molecule real-time sequencing (SMRT) revealed that the silage was enriched in the lactic acid bacteria (LAB), Lactobacillus rhamnosus after wilting, which showed a positive correlation with CP and lactic acid content. We conclude that wilting may help preserve paper mulberry silage, facilitating its use as a new fodder resource. Moreover, L. rhamnosus has the potential to be developed as a new inoculant for the modulation in wilted silages, particularly paper mulberry silage.
中文翻译：
对桑椹青贮发酵质量至关重要的微生物
青贮饲料质量仍然是农业中的一个重要问题，适用于动物饲料的产品范围也受到限制。因此，我们探索了对桑椹青贮发酵质量至关重要的微生物。在两个切割时间收获低（未枯萎）和高（枯萎）干物质（DM）纸桑。这些分别被青贮 0、3、7、14 和 56 天。与未枯萎的青贮相比，枯萎显着降低( p < 0.05)青贮pH值、氨氮浓度和酵母菌数，但增加( p < 0.05)乳酸含量。此外， 在枯萎的青贮饲料中也观察到较高的（ p < 0.05）粗蛋白（CP）含量。下一代测序 (NGS) 分析表明，萎蔫降低了肠杆菌，同时增加乳酸杆菌。单分子实时测序（SMRT）显示青贮青贮在萎蔫后富含乳酸菌（LAB）、鼠李糖乳杆菌，与CP和乳酸含量呈正相关。我们得出结论，枯萎可能有助于保存桑椹青贮，促进其作为新的饲料资源使用。此外，L . 鼠李糖有可能被开发为一种新的接种剂，用于调节枯萎的青贮饲料，特别是桑树青贮饲料。
更新日期：2021-06-15
中文翻译：
对桑椹青贮发酵质量至关重要的微生物
青贮饲料质量仍然是农业中的一个重要问题，适用于动物饲料的产品范围也受到限制。因此，我们探索了对桑椹青贮发酵质量至关重要的微生物。在两个切割时间收获低（未枯萎）和高（枯萎）干物质（DM）纸桑。这些分别被青贮 0、3、7、14 和 56 天。与未枯萎的青贮相比，枯萎显着降低( p < 0.05)青贮pH值、氨氮浓度和酵母菌数，但增加( p < 0.05)乳酸含量。此外， 在枯萎的青贮饲料中也观察到较高的（ p < 0.05）粗蛋白（CP）含量。下一代测序 (NGS) 分析表明，萎蔫降低了肠杆菌，同时增加乳酸杆菌。单分子实时测序（SMRT）显示青贮青贮在萎蔫后富含乳酸菌（LAB）、鼠李糖乳杆菌，与CP和乳酸含量呈正相关。我们得出结论，枯萎可能有助于保存桑椹青贮，促进其作为新的饲料资源使用。此外，L . 鼠李糖有可能被开发为一种新的接种剂，用于调节枯萎的青贮饲料，特别是桑树青贮饲料。
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