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Assessing conservation of alternative splicing with evolutionary splicing graphs
Genome Research ( IF 6.2 ) Pub Date : 2021-08-01 , DOI: 10.1101/gr.274696.120
Diego Javier Zea 1 , Sofya Laskina 2 , Alexis Baudin 3 , Hugues Richard 1, 2 , Elodie Laine 1
Affiliation  

Understanding how protein function has evolved and diversified is of great importance for human genetics and medicine. Here, we tackle the problem of describing the whole transcript variability observed in several species by generalizing the definition of splicing graph. We provide a practical solution to construct parsimonious evolutionary splicing graphs where each node is a minimal transcript building block defined across species. We show a clear link between the functional relevance, tissue regulation, and conservation of alternative transcripts on a set of 50 genes. By scaling up to the whole human protein-coding genome, we identify a few thousand genes where alternative splicing modulates the number and composition of pseudorepeats. We have implemented our approach in ThorAxe, an efficient, versatile, robust, and freely available computational tool.

中文翻译:

用进化剪接图评估选择性剪接的保守性

了解蛋白质功能如何进化和多样化对于人类遗传学和医学非常重要。在这里,我们通过推广剪接图的定义来解决描述在几个物种中观察到的整个转录本变异性的问题。我们提供了一个实用的解决方案来构建简约的进化剪接图,其中每个节点都是跨物种定义的最小转录构建块。我们展示了一组 50 个基因的功能相关性、组织调节和替代转录本的保存之间的明确联系。通过扩展到整个人类蛋白质编码基因组,我们鉴定了数千个基因,其中选择性剪接调节假重复的数量和组成。我们已经在 ThorAxe 中实现了我们的方法,ThorAxe 是一种高效、多功能、强大且免费的计算工具。
更新日期:2021-08-02
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