Within the nucleus, messenger RNA is generated and processed in a highly organized and regulated manner. Messenger RNA processing begins during transcription initiation and continues until the RNA is translated and degraded. Processes such as 5′ capping, alternative splicing, and 3′ end processing have been studied extensively with biochemical methods and more recently with single-molecule imaging approaches. In this review, we highlight how imaging has helped understand the highly dynamic process of RNA processing. We conclude with open questions and new technological developments that may further our understanding of RNA processing.

中文翻译：

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细胞核内 RNA 加工的成像组织


在细胞核内，信使 RNA 以高度组织和调节的方式生成和加工。信使 RNA 加工在转录起始期间开始，并持续到 RNA 被翻译和降解。诸如 5' 封端、选择性剪接和 3' 末端加工等过程已通过生化方法以及最近的单分子成像方法进行了广泛研究。在这篇综述中，我们重点介绍了成像如何帮助理解 RNA 加工的高度动态过程。最后，我们提出了一些悬而未决的问题和新技术的发展，这可能会进一步加深我们对 RNA 加工的理解。