Journal of Advanced Research ( IF 11.4 ) Pub Date : 2021-06-15 , DOI: 10.1016/j.jare.2021.06.010 Ana I Freitas 1 , Lucília Domingues 1 , Tatiana Q Aguiar 1
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Background
The potential applications of protein-engineered functional materials are so wide and exciting that the interest in these eco-friendly advanced materials will further expand in the future. Tag-mediated protein purification/immobilization technologies have emerged as green and cost-effective approaches for the fabrication of such materials. Strategies that combine the purification and immobilization of recombinant proteins/peptides onto/into natural, synthetic or hybrid materials in a single-step are arising and attracting increasing interest.
Aim of Review
This review highlights the most significant advances of the last 5 years within the scope of tag-mediated protein purification/immobilization and elucidates their contributions for the development of efficient single-step purification and immobilization strategies. Recent progresses in the field of protein-engineered materials created using innovative protein-tag combinations and future opportunities created by these new technologies are also summarized and identified herein.
Key Scientific Concepts of Review
Protein purification/immobilization tags present a remarkable ability to establish specific non-covalent/covalent interactions between solid materials and biological elements, which prompted the creation of tailor-made and advanced functional materials, and of next-generation hybrid materials. Affinity tags can bind to a wide range of materials (of synthetic, natural or hybrid nature), being most suitable for protein purification. Covalently binding tags are most suitable for long-term protein immobilization, but can only bind naturally to protein-based materials. Hybrid affinity-covalently binding tags have allowed efficient one-step purification and immobilization of proteins onto different materials, as well as the development of innovative protein-engineered materials. Self-aggregating tags have been particularly useful in combination with other tags for generating protein-engineered materials with self-assembling, flexible and/or responsive properties. While these tags have been mainly explored for independent protein purification, immobilization or functionalization purposes, efficient strategies that combine tag-mediated purification and immobilization/functionalization in a single-step will be essential to guarantee the sustainable manufacturing of advanced protein-engineered materials.
中文翻译：
标签介导的重组蛋白单步纯化和固定化，用于蛋白质工程先进材料
背景
蛋白质工程功能材料的潜在应用是如此广泛和令人兴奋，以至于人们对这些环保先进材料的兴趣在未来将进一步扩大。标签介导的蛋白质纯化/固定技术已成为制造此类材料的绿色且经济有效的方法。将重组蛋白/肽的纯化和固定化结合到天然、合成或杂化材料上的单一步骤的策略正在兴起并吸引了越来越多的兴趣。
审查目的
本综述强调了过去 5 年标签介导的蛋白质纯化/固定化领域最重要的进展，并阐明了它们对开发高效的单步纯化和固定化策略的贡献。本文还总结和确定了使用创新蛋白质标签组合创建的蛋白质工程材料领域的最新进展以及这些新技术创造的未来机会。
审查的关键科学概念
蛋白质纯化/固定标签具有在固体材料和生物元件之间建立特定非共价/共价相互作用的卓越能力，这促进了定制和先进功能材料以及下一代混合材料的创建。亲和标签可以结合多种材料（合成的、天然的或混合性质的），最适合蛋白质纯化。共价结合标签最适合长期蛋白质固定，但只能自然结合到基于蛋白质的材料。混合亲和共价结合标签可以实现蛋白质的高效一步纯化和固定到不同材料上，以及创新蛋白质工程材料的开发。自聚集标签与其他标签结合特别适用于生成具有自组装、柔性和/或响应特性的蛋白质工程材料。虽然这些标签主要用于独立的蛋白质纯化、固定化或功能化目的，但将标签介导的纯化和固定化/功能化结合在一个步骤中的有效策略对于保证先进蛋白质工程材料的可持续制造至关重要。
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