The baculovirus Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) is pathogenic to Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae) larvae, known as soybean looper, which is an important pest of soybean and bean. In this study, some parameters were tested to overcome the difficulties in the in vivo production of ChinNPV aiming to increase its use as a biopesticide. First, different combinations of larval instars (3rd and 4th instars), larval incubation temperatures (23 °C and 26 °C), and rearing densities (individually and 10 larvae/cup) were compared for larval weight and the production of occlusion bodies (OBs). A positive correlation (p< 0.001) was observed for OB production and larval weight. Fourth instar larvae produced more OBs than third instar larvae (p<0.05); however, no significant differences in OBs/larva (p>0.05) were observed for larvae kept in groups or individually. Therefore, a second assay was performed using fourth instar larvae incubated at 26 °C and two larval densities (10 larvae/cup and 40 larvae/cup). The losses of insects and OB production were evaluated as well as the influence of storage temperatures post-mortem (−20 °C, 4 °C, and 15 °C) in the OB yield. As expected, insect losses due to cannibalism or microbial contamination were greater (p<0.05) with the increase in larval density, although no difference was observed in OBs/larva (p>0.05). In addition, the storage temperature post-mortem did not influence the OB yield (p>0.05). The average production of ChinNPV OBs was 3×10¹⁰ OBs/40 larvae cup. The results demonstrate the viability of rearing C. includens in groups to enhance the mass production and reduce virus production costs.

杆状病毒Chrysodeixis includens 核多角体病毒(ChinNPV) 对Chrysodeixis includens具有致病性(Walker)（鳞翅目：夜蛾科）幼虫，俗称黄螟，是大豆和豆类的重要害虫。在这项研究中，测试了一些参数以克服 ChinNPV 体内生产中的困难，旨在增加其作为生物农药的用途。首先，比较幼虫体重和包涵体产生的幼虫龄（第 3 和第 4 龄）、幼虫孵化温度（23°C 和 26°C）和饲养密度（单个和 10 只幼虫/杯）的不同组合。 OB）。观察到 OB 产量和幼虫体重呈正相关 (p < 0.001)。四龄幼虫比三龄幼虫产生更多的OB（p<0.05）；然而，对于成组或单独饲养的幼虫，未观察到 OB/幼虫的显着差异（p>0.05）。所以，使用在 26°C 下孵化的四龄幼虫和两种幼虫密度（10 只幼虫/杯和 40 只幼虫/杯）进行第二次测定。评估了昆虫和 OB 生产的损失以及死后储存温度（-20°C、4°C 和 15°C）对 OB 产量的影响。正如预期的那样，由于自相残杀或微生物污染导致的昆虫损失随着幼虫密度的增加而增加（p < 0.05），尽管在 OB/幼虫中没有观察到差异（p>0.05）。此外，死后储存温度不影响 OB 产量 (p>0.05)。ChinNPV OB 的平均产量为 3×10 和 15 °C) 的 OB 产率。正如预期的那样，由于自相残杀或微生物污染导致的昆虫损失随着幼虫密度的增加而增加（p < 0.05），尽管在 OB/幼虫中没有观察到差异（p>0.05）。此外，死后储存温度不影响 OB 产量 (p>0.05)。ChinNPV OB 的平均产量为 3×10 和 15 °C) 的 OB 产率。正如预期的那样，由于自相残杀或微生物污染导致的昆虫损失随着幼虫密度的增加而增加（p < 0.05），尽管在 OB/幼虫中没有观察到差异（p>0.05）。此外，死后储存温度不影响 OB 产量 (p>0.05)。ChinNPV OB 的平均产量为 3×10¹⁰ OB/40 幼虫杯。结果证明了成群饲养C.includens以提高大规模生产和降低病毒生产成本的可行性。