Abstract

A novel octahydroindolizine alkaloid, named dendrocrepidamine (1) with an unusual 18,19,19′-cyclopropanone-dendrocrepine skeleton, was isolated from the ethanol extract of the roots of Dendrobium crepidatum, along with six known compounds (2–7). The structure of 1 was elucidated through HR-ESIMS, NMR spectroscopic data and computational calculations. All compounds were examined for their inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW264.7 cells with IC₅₀ values in the range of 3.04–54.89 µM. In vivo, crepidatin (6) (80, 40 and 10 mg/kg) showed a significant protective effect against LPS-induced acute lung injury (ALI) in mice.

摘要

一种新的八氢中生物碱，命名dendrocrepidamine（1）配有一个不寻常的18,19,19'-环丙酮-dendrocrepine骨架，从根的乙醇提取物中分离玫瑰石斛，具有六个已知化合物（沿2 - 7）。通过HR-ESIMS、NMR光谱数据和计算计算阐明了1的结构。检查了所有化合物对 RAW264.7 细胞中脂多糖 (LPS) 诱导的一氧化氮 (NO) 产生的抑制作用，IC ₅₀值在 3.04–54.89 µM 范围内。在体内，crepidatin ( 6 )) (80、40 和 10 mg/kg) 在小鼠中对 LPS 诱导的急性肺损伤 (ALI) 显示出显着的保护作用。