Citrullus colocynthis has been used for treating ailments since ages. The ability of plant extracts obtained using various solvents to exhibit pharmacological effects was evaluated. To check for the antioxidant potential of whole plant and fruits of C. colocynthis, conventional antioxidant assays such as DPPH radical scavenging capacity were carried out, total content of flavonoids and phenolics was determined, and reducing power was estimated. The antimicrobial activity was evaluated using 96-well plate technique and Alamar Blue assay and the potential of plant extracts as antioxidant agents was evaluated using oxidative burst assay with chemiluminescent indicators. The highest phenolic content was obtained using 50% ethyl acetate, while highest total flavonoid content was provided by extraction with methanol. Aqueous extracts showed highest DPPH radical scavenging activity, while methanolic extracts showed maximum reducing power potential at different concentrations. Whole plant extracts of C. colocynthis showed higher antioxidant potential than fruit extracts only. For both in-vitro and in-vivo anti-inflammatory activity, the maximum inhibition of reactive oxygen species (ROS) was produced by 50% methanol whole plant extract. Among the five test bacterial strains used, namely Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi, the antibacterial activity of extracts showed more pronounced growth inhibition of S. aureus and minimum effect upon E. coli. All extracts tested rendered zero potential as antiglycation agents.

Citrullus colocynthis 自古以来就被用于治疗疾病。评估了使用各种溶剂获得的植物提取物表现出药理作用的能力。检查C. colocynthis全株和果实的抗氧化潜力, 进行了常规的抗氧化试验，如 DPPH 自由基清除能力，测定了黄酮类和酚类的总含量，并估计了还原能力。使用 96 孔板技术和 Alamar Blue 测定法评估抗微生物活性，使用化学发光指示剂的氧化爆发测定法评估植物提取物作为抗氧化剂的潜力。使用 50% 乙酸乙酯获得最高酚类含量，而使用甲醇提取提供最高总黄酮含量。水提取物显示出最高的 DPPH 自由基清除活性，而甲醇提取物在不同浓度下显示出最大的还原能力。C. colocynthis 的全植物提取物显示出比仅水果提取物更高的抗氧化潜力。对于体外和体内抗炎活性，50% 甲醇全植物提取物对活性氧 (ROS) 的最大抑制作用。在所使用的五种试验菌株中，即大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌、铜绿假单胞菌和伤寒沙门氏菌，提取物的抗菌活性对金黄色葡萄球菌的生长抑制更为显着，对大肠杆菌的影响最小。测试的所有提取物作为抗糖化剂的潜力为零。