In this study, we developed a highly sensitive aptasensor based on polyaniline electro-polymerization, new nanomembrane composite, and aptamer (PANI-MWCNT@Chs-APT) for the electrochemical detection of estradiol valerate in real samples. The high sensitivity and selectivity of the aptasensor were demonstrated by evaluating its response to some potential interference having structural similarities. The selectivity of this aptasensor showed high selectivity for estradiol valerate among other interfering hormones. Electrochemical impedance spectroscopy combined with the standard added method used to characterize the modified gold electrodes as well as for the detection of estradiol valerate both in pharmaceutical and in women’s blood. It showed that the response of the aptasensor rose with increasing estradiol valerate concentration, which highlighted the good sensitivity of the aptasensor to estradiol valerate with a very low limit of detection of 0.56 fM. This was very promising for rapid analysis of estradiol valerate detection in pharmaceutical and biological samples.

Graphic abstract

中文翻译：

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纳米复合材料修饰的新型适体传感器在药物和真实生物样品中选择性测定戊酸雌二醇中的应用

在这项研究中，我们开发了一种基于聚苯胺电聚合、新型纳米膜复合材料和适体 (PANI-MWCNT@Chs-APT) 的高灵敏度适体传感器，用于电化学检测实际样品中的戊酸雌二醇。通过评估适体传感器对某些具有结构相似性的潜在干扰的响应，证明了适体传感器的高灵敏度和选择性。这种适体传感器的选择性显示出对戊酸雌二醇和其他干扰激素的高选择性。电化学阻抗谱与标准添加方法相结合，用于表征改性金电极以及检测药物和女性血液中的戊酸雌二醇。结果表明适体传感器的响应随着戊酸雌二醇浓度的增加而上升，这突出了适体传感器对戊酸雌二醇的良好灵敏度，检测限非常低，为 0.56 fM。这对于快速分析药物和生物样品中的戊酸雌二醇检测非常有希望。

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