Abstract

Purpose

Recently, therapeutic effects of extremely low-frequency electromagnetic field (ELF-EMF) as complementary and alternative medicine, used in the oncology field to control disease symptoms. Micro RNAs (miRs) are responsible for the post-transcriptional regulation of gene expression in the cell. This study aimed to evaluate the expression changes of miR-144 and miR-375 in the human gastric adenocarcinoma cell line (AGS) under the exposure of ELF-EMF.

Materials and methods

AGS cells were exposed to magnetic flux densities of 0.2 and 2 mT for 18 h, continuously and discontinuously (1.5 h on/1.5 h off). Cell viability was evaluated by MTT assay. Changes of miR-144 expression levels in AGS cells immediately after exposure and 18 and 36 h after the exposure cut-off was calculated by QRT-PCR.

Results

The cell viability of AGS cells was decreased under the exposure of 0.2 and 2 mT EMFs when compared to the control. Up-regulation of miR-144 and miR-375 were observed in AGS cells under the exposure of magnetic fields.

Conclusions

The results indicated that the miR levels were significantly decreased 18 and 36 h after finishing the exposure, but not reached the normal range. The results of this investigation indicated that weak and moderate intermittent 50 Hz ELF-EMFs can induce changes in miRNA expression.

中文翻译：

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暴露于极低频电磁场后人胃癌细胞系中 miR-144 和 miR-375 的上调

 抽象的

 目的

最近，极低频电磁场（ELF-EMF）作为补充和替代医学的治疗作用，用于肿瘤领域来控制疾病症状。微小RNA (miR) 负责细胞中基因表达的转录后调控。本研究旨在评估ELF-EMF暴露下人胃腺癌细胞系（AGS）中miR-144和miR-375的表达变化。

 材料和方法

AGS 细胞连续或间断（1.5 小时开启/1.5 小时关闭）暴露于 0.2 和 2 mT 的磁通量密度下 18 小时。通过MTT测定评估细胞活力。通过QRT-PCR计算暴露后立即以及暴露截止后18和36小时AGS细胞中miR-144表达水平的变化。

 结果

与对照相比，AGS 细胞在 0.2 和 2 mT EMF 暴露下的细胞活力下降。在磁场暴露下，AGS 细胞中观察到 miR-144 和 miR-375 上调。

 结论

结果表明，暴露后18和36小时，miR水平显着下降，但未达到正常范围。这项研究的结果表明，弱和中度间歇性 50 Hz ELF-EMF 可以诱导 miRNA 表达的变化。