Selecting an appropriate separation technique is essential for the application of in situ product removal (ISPR) technology in biological processes. In this work, a three-stage systematic design method is proposed as a guide to integrate ionic liquid (IL)-based separation techniques into ISPR. This design method combines the selection of a suitable ISPR processing scheme, the optimal design of an IL-based liquid–liquid extraction (LLE) system followed by process simulation and evaluation. As a proof of concept, results for a conventional acetone–butanol–ethanol fermentation are presented (40,000 ton/year butanol production). In this application, ILs tetradecyl(trihexyl)phosphonium tetracyanoborate ([TDPh][TCB]) and tetraoctylammonium 2-methyl-1-naphthoate ([TOA] [MNaph]) are identified as the optimal solvents from computer-aided IL design (CAILD) method and reported experimental data, respectively. The dynamic simulation results for the fermentation process show that, the productivity of IL-based in situ (fed-batch) process and in situ (batch) process is around 2.7 and 1.8fold that of base case. Additionally, the IL-based in situ (fed-batch) process and in situ (batch) process also have significant energy savings (79.6% and 77.6%) when compared to the base case.

中文翻译：

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基于离子液体的原位产物去除设计以丙酮-丁醇-乙醇发酵为例

选择合适的分离技术对于在生物过程中应用原位产物去除 (ISPR) 技术至关重要。在这项工作中，提出了一种三阶段系统设计方法作为将基于离子液体 (IL) 的分离技术集成到 ISPR 中的指南。这种设计方法结合了合适的 ISPR 处理方案的选择、基于 IL 的液液萃取 (LLE) 系统的优化设计以及过程模拟和评估。作为概念验证，展示了常规丙酮-丁醇-乙醇发酵的结果（40,000 吨/年的丁醇产量）。在这个应用程序中，ILs 十四烷基（三己基）鏻四氰基硼酸盐（[TDPh][TCB]）和 2-甲基-1-萘甲酸四辛基铵（[TOA] [MNaph]）被计算机辅助 IL 设计（CAILD）方法确定为最佳溶剂并已报道实验数据，分别。发酵过程的动态模拟结果表明，基于 IL 的原位（补料分批）工艺和原位（分批）工艺的生产率分别是基本情况的 2.7 倍和 1.8 倍。此外，与基本情况相比，基于 IL 的原位（分批补料）工艺和原位（分批）工艺也具有显着的节能效果（79.6% 和 77.6%）。是基本情况的 8 倍。此外，与基本情况相比，基于 IL 的原位（分批补料）工艺和原位（分批）工艺也具有显着的节能效果（79.6% 和 77.6%）。是基本情况的 8 倍。此外，与基本情况相比，基于 IL 的原位（分批补料）工艺和原位（分批）工艺也具有显着的节能效果（79.6% 和 77.6%）。