ABSTRACT

Colon cancer is a malignant tumor that seriously affects human health. Recently, studies revealed that the expression of MTBP enhanced the proliferation and metastasis of many types of cancer cells. And the data also showed that MTBP has the potential to regulate the expression of ZEB2. However, it is unclear whether MTBP can affect the proliferation, migration and invasion of colon cancer cells by modulating the expression of ZEB2. In this study, we established the MTBP overexpression and knockdown colon cancer cells with the transfection. Next, CCK-8 and transwell assays were carried out to determine the changes of the proliferation and invasion of colon cancer cells, respectively. After that, we overexpressed the ZEB2 in these MTBP knockdown colon cancer cells. Finally, the invasion and migration of these cells were detected with the same methods. We revealed that overexpression of MTBP enhanced the proliferation and invasion of colon cancer cells. Moreover, suppression of MTBP repressed the proliferation, migration and invasion of colon cancer cells. Furthermore, MTBP promoted the expression of ZEB2. The overexpression of ZEB2 abolished the MTBP knockdown induced inhibition of the migration and invasion of colon cancer cells. These results implied that MTBP enhanced the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2.

摘要

结肠癌是严重影响人类健康的恶性肿瘤。最近，研究表明MTBP的表达增强了多种癌细胞的增殖和转移。并且数据还表明MTBP具有调节ZEB2表达的潜力。然而，目前尚不清楚MTBP是否可以通过调节ZEB2的表达来影响结肠癌细胞的增殖、迁移和侵袭。在这项研究中，我们通过转染建立了 MTBP 过表达和敲低结肠癌细胞。接下来，分别进行CCK-8和transwell实验，以测定结肠癌细胞增殖和侵袭的变化。之后，我们在这些 MTBP 敲低的结肠癌细胞中过度表达了 ZEB2。最后，用相同的方法检测这些细胞的侵袭和迁移。我们发现MTBP的过表达增强了结肠癌细胞的增殖和侵袭。此外，MTBP 的抑制抑制了结肠癌细胞的增殖、迁移和侵袭。此外，MTBP 促进了 ZEB2 的表达。ZEB2的过表达消除了MTBP敲低诱导的结肠癌细胞迁移和侵袭的抑制。这些结果暗示MTBP通过激活ZEB2的表达增强结肠癌细胞的增殖、迁移和侵袭。MTBP促进了ZEB2的表达。ZEB2的过表达消除了MTBP敲低诱导的结肠癌细胞迁移和侵袭的抑制。这些结果暗示MTBP通过激活ZEB2的表达增强结肠癌细胞的增殖、迁移和侵袭。MTBP促进了ZEB2的表达。ZEB2的过表达消除了MTBP敲低诱导的结肠癌细胞迁移和侵袭的抑制。这些结果暗示MTBP通过激活ZEB2的表达增强结肠癌细胞的增殖、迁移和侵袭。