Tropospheric ozone (O3) concentration has been increasing. The aim of this study was to compare the effect of O3 exposure on monoterpene emission rate and the components of Japanese cedar (Cryptomeria japonica) between three major gene pools identified by population genetic structure analysis. At the O3-FACE (Free-Air O3 and CO2 Enrichment) facility, 1-year saplings of three clones of Japanese cedar (Donden, Kawazu, and Yakushima) were exposed to ambient double concentrations of O3. After 15 months of O3 exposure experiment, monoterpene emission characteristics were evaluated by branch enclosure method using cutting samples of current-year leaves. Basal biogenic volatile organic compound emission rate (Es) was higher in the O3 exposure plots, and two-way analysis of variance revealed an effect of the O3 exposure (p <0.05) and an interaction between the O3 exposure and clone (p < 0.05). Especially, high Es values were observed in the Yakushima after the O3 exposure. In addition, the coefficient β related to temperature sensitivity increased in the O3 exposure plots (p < 0.05). Our findings supported the fact that there are large inter-clone variations in C. japonica. It is important to evaluate the differences in clones considering the genetic diversity and to clarify the mechanism of the differences after long-term moderate O3 exposure.

中文翻译：

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代表Cryptomeria japonica主要基因库的三个克隆之间臭氧暴露后单萜排放特征的差异

对流层臭氧 (O3) 浓度一直在增加。本研究的目的是比较 O3 暴露对通过种群遗传结构分析确定的三个主要基因库之间的单萜排放率和日本雪松（Cryptomeria japonica）成分的影响。在 O3-FACE（自由空气 O3 和 CO2 富集）设施中，三个日本雪松无性系（Donden、Kawazu 和 Yakushima）的 1 年树苗暴露于双倍浓度的 O3 环境中。经过 15 个月的 O3 暴露实验，采用枝条封闭法使用当年叶片的切割样品评估单萜排放特性。O3 暴露地块中的基础生物挥发性有机化合物排放率 (Es) 较高，方差的双向分析显示 O3 暴露的影响 (p < 0. 05) 以及 O3 暴露和克隆之间的相互作用 (p < 0.05)。特别是，在 O3 暴露后在屋久岛观察到高 Es 值。此外，在 O3 暴露图中，与温度敏感性相关的系数 β 增加（p < 0.05）。我们的研究结果支持这样一个事实，即在 C. japonica 中存在较大的克隆间变异。考虑到遗传多样性评估克隆的差异并阐明长期中度 O3 暴露后差异的机制很重要。粳稻。考虑到遗传多样性评估克隆的差异并阐明长期中度 O3 暴露后差异的机制很重要。粳稻。考虑到遗传多样性评估克隆的差异并阐明长期中度 O3 暴露后差异的机制很重要。