Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA-DR), measured by standardized flow cytometry procedure, is a reliable indicator of immunosuppression in severely injured intensive care unit patients. As such, it is used as stratification criteria in clinical trials evaluating novel immunostimulating therapies. Preanalytical constraints relative to the short delay between blood sampling and flow cytometry staining have nevertheless limited its use in multicentric studies. The objective of the present work was to compare mHLA-DR expression between whole blood samples simultaneously drawn in EDTA or Cyto-Chex BCT tubes.

中文翻译：

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用于评估临床样本中单核细胞 HLA-DR 表达的稳定取样管的双中心评估

通过标准化流式细胞术程序测量的循环单核细胞 (mHLA-DR) 上人类白细胞抗原 DR 的表达减少是重症监护病房患者免疫抑制的可靠指标。因此，它被用作评估新型免疫刺激疗法的临床试验的分层标准。然而，与血液采样和流式细胞术染色之间的短暂延迟相关的分析前限制限制了其在多中心研究中的使用。本工作的目的是比较在 EDTA 或 Cyto-Chex BCT 管中同时抽取的全血样本之间的 mHLA-DR 表达。