ABSTRACT

SARD1 and CBP60g are two master regulators in plant immunity. They are required for the constitutive defense responses in the Arabidopsis snc2-1D mutant, which carries a gain-of-function mutation in a receptor-like protein. Here we report that WRKY54 and WRKY70 are required for activation of SARD1 and CBP60g expression and defense responses in snc2-1D. In addition, the induction of SARD1 and CBP60g by the bacterial pathogen Pseudomonas syringae pv. maculicola is significantly reduced in sid2 wrky54 wrky70 triple mutants compared to the sid2 single mutants, suggesting that WRKY54 and WRKY70 positively regulate the SID2-independent expression of SARD1 and CBP60g during pathogen infection. Our study revealed WRKY54 and WRKY70 as positive regulators of SARD1 and CBP60g expression in plant defense.

摘要

SARD1 和 CBP60g 是植物免疫的两个主要调节因子。它们是拟南芥snc2-1D突变体的组成性防御反应所必需的，该突变体在受体样蛋白中携带功能获得突变。在这里，我们报告 WRKY54 和 WRKY70 是 snc2-1D 中SARD1和CBP60g表达和防御反应的激活所必需的。此外，细菌病原体丁香假单胞菌pv诱导SARD1和CBP60g 。与 sid2 相比， sid2 wrky54 wrky70三重突变体中的maculicola显着减少单个突变体，表明WRKY54和WRKY70在病原体感染期间正向调节SID2非依赖性 SARD1 和 CBP60g 的表达。我们的研究揭示了 WRKY54 和 WRKY70 作为植物防御中SARD1和CBP60g表达的正调节因子。