In sorghum [Sorghum bicolor (L.) Moench], development of male-sterile versions (A-lines) of the seed parents (B-lines) is a tedious and time-consuming process limiting opportunities for the improvement of new seed parents. Assessment of the value of a potential new seed parent requires testcross hybrid evaluations, but because of male fertility of these lines, conventional methods are inadequate to generate hybrid seed required for testing. Mechanisms to reduce the number of lines that require sterilization would be beneficial. The chemical gametocide trifluoromethanesulfonamide (TFMSA) was evaluated for its potential applicability to generate testcross hybrid seed. To study the effect on inbred line productivity, foliar applications of 25 mg of TFMSA were made to a select set of male-fertile B-lines and were compared to the corresponding untreated male-sterile A-line. Trifluoromethanesulfonamide induced near-complete male-sterility and allowed for testcross hybrid seed production across a range of genotypes. Comparison to untreated B-lines indicated an average 3-d delay in flowering and a 13-cm reduction in plant height. Furthermore, average seed weight per panicle (productivity) was reduced by 8 g. Comparison of testcross hybrids produced by TFMSA induced male-sterile lines and cytoplasmic male-sterile lines did not reveal any significant and meaningful differences between the hybrids derived from both sterility systems. Moreover, inbred lines produced hybrids that ranked consistently in both systems. These results show TFMSA can affect productivity of treated inbred lines, but not the derived hybrid, which demonstrates that TFMSA can be used to produce hybrids for evaluation prior to investment in sterilizing of these lines.

中文翻译：

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三氟甲磺酰胺对高粱自交系及其衍生杂种的影响分析

在高粱中 [ Sorghum bicolor(L.) Moench]，种子亲本（B 系）的雄性不育版本（A 系）的开发是一个繁琐且耗时的过程，限制了改进新种子亲本的机会。评估潜在新种子亲本的价值需要进行测试杂交评估，但由于这些品系的雄性育性，常规方法不足以产生测试所需的杂交种子。减少需要消毒的生产线数量的机制将是有益的。评估了化学杀配子三氟甲磺酰胺 (TFMSA) 的潜在适用性，以产生 testcross 杂交种子。为了研究对近交系生产力的影响，将 25 mg TFMSA 叶面施用到一组精选的雄性可育 B 系，并与相应的未处理的雄性不育 A 系进行比较。三氟甲磺酰胺诱导了近乎完全的雄性不育，并允许跨一系列基因型进行测试杂交种子生产。与未经处理的 B 系相比，开花平均延迟 3 天，植物高度减少 13 厘米。此外，每穗的平均种子重量（生产力）减少了 8 克。由 TFMSA 诱导的雄性不育系和细胞质雄性不育系产生的测试杂交杂种的比较未揭示源自两种不育系统的杂种之间的任何显着且有意义的差异。此外，近交系产生的杂种在两个系统中的排名一致。这些结果表明 TFMSA 可以影响处理过的自交系的生产力，但不会影响派生的杂种，