Greenbug (Schizaphisgraminum Rondani) is one of the aphids causing significant cereal yield losses worldwide, and greenbug Biotype H is virulent to all known greenbug resistance genes in barley (Hordeum L.). Wild barley (Hordeum vulgare ssp. spontaneum) accession WBDC336 (PI 682028) exhibits resistance to greenbug Biotypes C, E, H, I, WY81, WY12 MC, and WY86. To investigate the genetics of greenbug resistance in WBDC336, a recombinant inbred line (RIL) population was developed from the cross Weskan × WBDC336. The RIL population was evaluated for responses to greenbug Biotype E and genotyped using genotyping-by-sequencing. Among 67,238 single nucleotide polymorphisms (SNPs) identified, 3,907 high quality SNPs were selected for gene mapping after several filtering steps. Linkage analysis positioned the greenbug resistance gene to a 1.14-Mb interval in the terminal region of the long arm of chromosome 3H in WBDC336, where the greenbug resistance gene Rsg1 resides. Greenbug assays and an allelism test indicated that the greenbug resistance gene in WBDC336 is likely an allele at the Rsg1 locus and was given the allele designation Rsg1.a3. Two Kompetitive allele-specific polymerase chain reaction (KASP) markers were developed from SNPs closely linked to Rsg1.a3, and these markers can be used to tag both alleles at the Rsg1 locus in breeding program utilizing marker-assisted selection.

中文翻译：

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Rsg1.a3：一种新的等位基因，在大麦中的 Rsg1 基因座赋予对绿蚧生物型 H 的独特抗性。自发性

绿蚧 ( Schizaphisgraminum Rondani) 是导致世界范围内严重谷物减产的蚜虫之一，绿蚧 H 对大麦 ( Hordeum L.) 中所有已知的绿蚧抗性基因都具有毒性。野大麦（Hordeum vulgare ssp. spontaneum) 加入 WBDC336 (PI 682028) 表现出对青虫生物型 C、E、H、I、WY81、WY12 MC 和 WY86 的抗性。为了研究 WBDC336 中青虫抗性的遗传学，从杂交 Weskan × WBDC336 开发了重组自交系 (RIL) 种群。评估了 RIL 群体对 greenbug 生物型 E 的反应，并使用基因分型测序进行基因分型。在鉴定的 67,238 个单核苷酸多态性 (SNP) 中，经过几个过滤步骤后，选择了 3,907 个高质量 SNP 用于基因定位。连锁分析将绿虫抗性基因定位到 WBDC336 染色体 3H 长臂末端区域的 1.14-Mb 间隔，其中绿虫抗性基因Rsg1居住。Greenbug 检测和等位基因测试表明 WBDC336 中的 greenbug 抗性基因可能是Rsg1基因座的等位基因，并被指定为等位基因Rsg1.a3。两个竞争性等位基因特异性聚合酶链反应 (KASP) 标记是从与Rsg1.a3紧密连锁的 SNP 开发的，这些标记可用于在育种程序中利用标记辅助选择标记Rsg1基因座上的两个等位基因。